FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136811
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT49640.4366600956535431No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25640.22554320814981557No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA23170.20381576181089026No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC20430.17971325048754808No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC18930.1665184450185651No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG18920.1664304796487719No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18760.16502303373208035No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA18030.15860156173717532No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT17740.15605056601317194No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT16300.14338355276294829No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG16000.1407445916691517No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA15880.13968900723163305No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG15390.13537870411176528No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT14870.13080450488251785No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA14590.1283414745283077No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA14280.1256145480647179No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT13850.12183203716360942No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13800.12139221031464334No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13690.1204245912469179No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13560.11928104143960605No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT13490.11866528385105352No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG12770.1123317772259417No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG12700.11171601963738916No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA12590.11074840056966373No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA12320.10837333558524681No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG12250.10775757799669428No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT12080.10626216671020952No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC12040.10591030523103663No Hit
GTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCTGTATCAGTCT12020.1057343744914502No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT11940.10503065153310445No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA11920.10485472079351801No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT11790.10371117098620615No Hit
GTGTAACTGTTATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAA11780.10362320561641293No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT11590.10195186359034175No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC11560.1016879674809621No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG11480.10098424452261634No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCACG100.0070910784144.99939145
GTAACAA15550.0124.962234
CACGCAC900.0120.843453
GAAACAC4250.0114.303694
CGAGCGG203.8662596E-4108.749535145
AAACACG4500.0107.953495
AACACGT1500.0101.508496
TAGAAAC5250.099.43692
GCACGCA1150.094.5731352
TAACAAG20600.093.976315
AGCACGC1200.090.6405641
TTTCCCG406.118171E-790.62462145
TAGTAAC23500.083.304852
ACGTGCA3700.080.344572
CACGTGC4400.079.104491
AGTAACA26750.074.26793
GGCTGGG300.001934492472.512451
CGGTGCA300.001935167772.506072
CGCACTT1600.072.506075
ACACGTG2000.072.4996957
Produced by FastQC (version 0.11.5)