FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226855
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT36730.2993833827143387No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT33670.27444155992354435No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT26690.21754812100859516No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23730.19342139046586598No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA23360.19040554914802482No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT22600.1842108480627295No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT21840.17801614697743418No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG21120.1721474827913649No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT21090.17190295511694537No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT20470.16684938317894127No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC19160.15617167472928747No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA19000.15486752713238322No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC18540.1511181027912834No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC17620.1436192541090838No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT17610.14353774488427726No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC17250.14060341279124267No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG17150.13978832054317747No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG16960.13823964527185365No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA16520.13465323938036688No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC15670.12772495527181288No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA15460.12601326155087603No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC15020.12242685565938924No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT14970.12201930953535667No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA14920.12161176341132407No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC14870.1212042172872915No Hit
AACCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATG14820.1207966711632589No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG14300.11655819147332No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT14260.11623215457409392No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC13950.11370536860509188No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC13820.11264574868260716No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT13300.10840726899266824No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG13290.10832575976786174No Hit
TCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAGATTTCTTTCT13180.10742915829499004No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT12950.10555444612444012No Hit
GCTTTATGGTCTGGCGGTTAAAGGACAATCTCATCTGAGAGGAGATACTG12690.10343520627947068No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC12690.10343520627947068No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA12630.10294615093063159No Hit
GTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTA12610.10278313248101853No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC12590.10262011403140552No Hit
AATATACTGTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAA12470.10164200333372729No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA12350.1006638926360491No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT12340.10058238341124257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGA100.0070852665145.039634
TATCGAG100.0070852665145.039632
CACGCAC4600.0122.9683763
GCACGCA4700.0121.8950042
CAGGCCG301.1019438E-7120.83188145
AGCACGC4750.0119.090021
AAACACG3250.0116.031715
GAAACAC3400.0110.912664
CGCACTT5400.0110.122685
CCCGCAC203.862068E-4108.779723
ACGCACT5400.0106.09384
AAGCGGT301.5974356E-596.6930852
CGGTGCC259.377075E-487.023772
AACACGA3300.085.705246
TAGAAAC4500.083.8006742
GCGGTGC1150.081.982261
CGGTGCG755.638867E-1177.354462
GTAGTAA11950.074.646871
GCACTTT10350.073.570826
CCCACTA300.0019359472.49912145
Produced by FastQC (version 0.11.5)