FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835842
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT21910.2621308811952498No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20400.244065265923464No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20350.24346706674227905No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA17580.2103268321046322No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT15670.1874756233833667No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA14960.17898119501054027No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA14700.17587055926837847No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT14000.167495770731789No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13860.16582081302447113No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT11940.14284996446696863No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC11800.14117500675965075No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC11800.14117500675965075No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA10690.1278949849373446No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC10110.12095587443559908No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT10080.12059695492688809No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC9980.11940055656451817No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA9950.1190416370558072No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT9520.11389712409761654No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC9500.11365784442514254No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC9460.11317928508019458No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC9430.11282036557148362No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG9390.11234180622653564No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT9010.10779549244952995No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA8960.10719729326834498No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC8850.10588125506973806No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT8590.10277061932757625No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA8420.1007367421115474No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT8370.10013854293036244No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC100.0070906556145.01
CGCTACG100.0070906556145.03
CGGTCAA100.0070906556145.05
AGCACGC3600.0112.777781
CACGCAC3550.0112.3239443
GCACGCA3650.0111.232882
GAAACAC2750.0105.4545444
CAACGCA1000.0101.59
AAACACG3050.095.081965
CGCACTT4450.091.235955
ACGCACT4400.090.624994
GCGGTGC750.087.01
CGGTGCG502.2682798E-887.02
CTACTTA353.4369463E-582.857146
ACAACGC1150.081.956528
TAGTAAC12400.075.4233862
GTAGTAA12700.075.354331
GAAGCGG1350.075.185191
GTAACAA13050.071.666674
ACGTGCA1150.069.3478242
Produced by FastQC (version 0.11.5)