FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020349
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT32060.3142062176765009No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20810.20394982501085412No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT18750.1837606544427446No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT18670.1829766089837889No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT17330.16984384754628074No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT16200.15876920543853132No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT15790.1547509724613833No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA15680.1536729099553192No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG15580.15269285313162456No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG15190.1488706315192155No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG15150.14847860878973762No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15070.14769456333078193No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG14810.14514641558917588No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14490.142010233753353No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT14060.1377959894114661No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA13770.13495382462275163No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA13430.13162163142218986No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13400.13132761437508145No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG13240.12975952345717004No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT13210.12946550641006166No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA13140.12877946663347542No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA13010.1275053927626724No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT12620.1236831711502633No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG12070.11829285861994279No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC12020.11780283020809547No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC11830.11594072224307567No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC11450.11221650631303604No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA11340.11113844380697194No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTGCTAGTTTTCCTTCT11250.11025639266564674No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT11210.10986436993616891No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG11020.10800226197114908No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA10790.10574813127665142No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT10470.10261194944082858No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC10260.10055383011106984No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA10230.10025981306396145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAG100.0070868996145.027255
TAGTGGG100.0070910547144.99883145
GTAACAA11000.0133.82064
CACGCAC950.0129.761233
AAACACG4200.0115.6765065
GAAACAC4300.0112.986344
GCACGCA1150.0107.194052
TAGAAAC4650.0106.0414352
AGCACGC1200.0102.727641
CGCACTT1300.094.8255165
AACACGT1850.090.152076
TAACAAG16700.088.579525
CCACGAC259.3871047E-486.9993145
TAGTAAC17050.085.9105762
CAGAGCG608.276402E-1084.599235
ACGTGCA2450.082.872722
AGCGTTT851.8189894E-1276.7640848
GAGCGTT851.8189894E-1276.7640847
ACAACAG2000.076.1393051
TACCTCT300.001934310972.5136264
Produced by FastQC (version 0.11.5)