FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031087
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC60350.5853046348174306No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA46280.4488467025575921No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT41450.4020029347669013No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG35570.3449757391956256No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT32420.3144254558538707No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG32260.3128736954301626No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA29390.285038992829897No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT28700.2783470260026555No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT26430.2563314249912956No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT25640.24866960789923642No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT23490.22781782720565769No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA21330.20686906148559722No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT21320.20677207645911547No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT20960.2032806155057721No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG20500.19881930428761102No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA19520.1893147716923984No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA18960.18388361020941976No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC18690.1812650144944122No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA15820.15343031189414666No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA15650.1517815664439567No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC15450.1498418659143215No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA15380.14916297072894916No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT15120.14664136004042336No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA14940.14489562956375165No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG14530.14091924347799942No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC14430.13994939321318184No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG14210.13781572263058306No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC14040.1361669771803931No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT13750.13335441141242205No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC13510.13102677077685976No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG13310.12908707024722452No Hit
TCTTTGGTCCATTGTCAAGAAAAGCCTTGCGAGGAACCCAATGGTGTTCT12950.1255956092938811No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA12930.12540163924091757No Hit
TTTTCATCCTGGGTGGCATGAACGGTCCCTCCAGAAAGAAGTCACTTCTC12690.12307399860535533No Hit
GTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAG12200.11832173230774899No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC12050.11686695691052258No Hit
AAGCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA12020.1165760018310773No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA11930.11570313659274145No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC11920.1156061515662597No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT11830.11473328632792383No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA11820.11463630130144208No Hit
CATATCAGCAAGTCCTCCAAGTGGGTCCCTATCCATTTTCATCCTGGGTG11740.11386042108958798No Hit
ACCCAATGGTGTTCTCGCAACTCTACGGTATCCTGCAGCAGGAAACGAAT11700.11347248098366094No Hit
ACTCTACGGTATCCTGCAGCAGGAAACGAATCCTTGCTGGCAATTCCTTA11170.10833227458012758No Hit
ATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGTCCATTGT11040.10707146923586468No Hit
AATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAA10900.10571367886512001No Hit
GTAAAAGATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAG10770.10445287352085712No Hit
GCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGCATATCCTTCGACTG10710.10387096336196655No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT10650.10328905320307598No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC10650.10328905320307598No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG10610.10290111309714893No Hit
GCACAGCTCATTGCCGGCTACATCGATATCATCCTGAAGAAGAAAAAAAG10450.10134935267344075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC18800.0120.335921
GCACGCA20050.0115.003572
ACGCACT20850.0110.9387364
CGCACTT21950.0108.682585
CACGCAC23050.0100.035643
GCGGTGC9750.091.47431
GCACTTC12750.090.424386
TAGTAAC7700.087.5771562
CACTTGA13050.083.888387
GCACTTG14650.081.666696
GAAGCGG6500.080.318891
ACTTCGG2450.079.897488
GTAGTAA8800.077.453991
TAACAAG8700.076.677365
CACTTCG2700.075.184737
CTTCGGT1700.072.4995659
CCCCGTT1850.070.5401159
AGTAACA9950.067.044533
GCACCAC36600.064.58556
CCACATT35700.062.9548039
Produced by FastQC (version 0.11.5)