FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214592
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT35500.2922792180419433No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT32730.2694732058172621No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT28770.23686966487511857No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA28750.23670500052692592No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC27720.22822478659500475No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT21130.1739678838655285No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA18850.15519614817156707No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT16550.13625974812941302No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT15860.1305788281167668No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA15580.12827352724206978No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT15530.12786186637158817No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA15410.1268738802824323No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC15160.12481557593002424No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC14550.1197933133101486No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT14440.11888765939508907No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG14230.1171586837390663No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA13910.11452405416798397No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG13210.10876080198124144No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT13190.10859613763304879No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA12850.10579684371377383No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA12820.10554984719148487No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG12750.10497352197281061No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG12530.10316221414269154No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA12450.10250355674992095No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC12170.10019825587522393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG100.007089381145.011415
CTAGGTT202.1308333E-6144.99947145
AAACACG4800.0116.3112265
AGCACGC5500.0116.01391
GCACGCA5500.0116.0091252
CCCGCAC256.4629094E-6116.0091253
CACGCAC5550.0114.9643
GAAACAC5150.0108.4065864
TAGAAAC6300.090.919852
CGCACTT7400.090.142235
ACGCACT7300.088.397364
AACACGT2650.087.5540546
CGGTGCC502.267916E-887.006842
TGCACGC259.387374E-486.99968145
TAGTAAC19200.082.701812
AACACGA4150.080.367766
ACAACGC1850.078.378098
ACGTGCA2250.077.339412
ACACGTG3100.074.838437
GCCCGCA300.001935238872.50572
Produced by FastQC (version 0.11.5)