FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218176
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT45190.37096445833771147No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT31230.2563668960806977No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT22710.18642626352842281No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21150.17362023221603448No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA20220.16598586739518756No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC20050.16459033834191447No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA18290.15014250814332247No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17910.14702309025953558No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC17400.1428365030997163No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG17090.14029171482610067No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG16880.13856782599558684No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT15960.13101555111905014No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA15200.1247767153514763No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA14940.12264237679941158No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14760.12116475780182831No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG14420.11837369969528212No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA14030.11517219186718505No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT12940.10622438793737522No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC12600.10343332983082905No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12490.10253034044341704No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG12320.10113481139014395No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT12260.10064227172428286No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA12220.10031391194704213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGACT100.007088554145.017074
AGCGGTT100.007091164144.999228
TGCAGCG100.007091164144.99922145
GTAACAA15550.0126.831654
GAAACAC6100.0121.243784
GCACGCA1050.0117.394782
TAGAAAC6900.0114.5424652
AAACACG6450.0113.54055
GTCGCAT203.8644506E-4108.762813
CACGCAC1150.0107.186553
AGCACGC1250.098.615661
AACACGT2450.088.7859656
CGCACTT1500.087.010255
CATAAGG703.092282E-1182.87031
TTTCCCG703.092282E-1182.856705145
TAACAAG24450.080.960465
AACACGA6450.080.939766
TAGTAAC25000.080.049422
ACACGTG2750.079.0904857
ACACGAG7150.073.00667
Produced by FastQC (version 0.11.5)