FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_AHA5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_AHA5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961442
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC12900.13417346028153546No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT12860.13375741854422835No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA11290.11742778035492521No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA10050.10453048649840552No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA9780.10172220477158268No Hit
ATATGATAGTGATGAACCAGAATTGAGGTCGCTAGCAAGTTGGATTCAGA9770.10161819433725593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1500.0106.343941
AGCGAAA3050.054.677581
GTAGTCG400.00604842854.3804246
GCAAAAG15250.051.349383
TACGACC450.00963522848.3381544
CTATTCG450.00963918948.3331269
GAGCAAA20750.039.136431
CGAAAGC4350.036.6703223
GCGGGTA850.002741991334.11759
TAGTACT1751.00824764E-733.146164
TCGTACT900.00362639132.2254332
CAAAAGC24250.031.992884
CTATGCT6700.030.301534
AAGCAGG31400.030.2466857
TAGGTCC4800.030.2113486
GTATAGA1703.0092178E-629.8559171
TTATACC1504.395542E-529.0028924
GTAGTAC2003.2512798E-729.0028923
CACGAGC1804.699874E-628.194323145
TCGATAG1555.496468E-528.0673162
Produced by FastQC (version 0.11.5)