FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061371
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA39910.3760230871203378No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG35320.33277713447983787No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT27110.25542435208800696No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT26180.24666210024581414No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG24360.22951446760840463No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC24120.22725324132654842No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA22440.211424657353555No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG22040.20765594688379463No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT21960.2069022047898426No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT21880.20614846269589052No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA20340.1916389273873132No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG18780.17694095655524789No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA18350.17288959280025554No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT18130.17081680204188734No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA17510.16497530081375883No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG16910.1593222351091183No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT16100.15169059640785362No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT15610.1470739260823972No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT15070.14198616694822075No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA14890.1402902472368286No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA14480.13642731900532426No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT14050.13237595525033188No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13580.1279477204483635No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA13450.12672288954569136No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA13380.1260633652134833No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG13310.12540384088127526No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA12960.12210621922023494No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG12960.12210621922023494No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC12940.12191778369674694No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA12860.12116404160279488No Hit
GTACGGGGACGGAGCTTCACCGACAGGGCAGCTCATTAAGGCCCTATAAG12780.12041029950884281No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT12520.11796063770349859No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG12460.11739533113303453No Hit
CCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAAC12400.11683002456257048No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC11840.11155382990490602No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC11750.11070587004920994No Hit
CAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA11690.11014056347874589No Hit
TCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATG11430.10769090167340167No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA11380.10721981286468162No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG11200.10552389315328947No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT10980.1034511023949213No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA10950.10316844910968927No Hit
GCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGTACAATTCAAG10740.1011898761130651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC100.0070899655145.006552
GAGCGAA1850.0125.4110641
CTCCGAT203.8654284E-4108.754911
CCGGATA203.8654284E-4108.754914
TTAGTAG650.0100.389151
GCAAAAG18250.093.757663
ACGGCCC259.385225E-487.003934
TACCGGA259.385225E-487.003932
AGCAGGC4000.086.999838
TAAACTA4050.080.559195
CTATTCG4150.076.867329
GCAGGGG7050.074.042419
CCGATGG300.001935428872.503273
CTCCCGG300.001935428872.503271
AGCGAAA3900.070.644211
TTTAAAC4850.067.271083
CGCTCCC651.3976933E-766.92616
CAAAAGC25750.066.44964
ATGGTAC1009.094947E-1265.2529453
CAGGGAC1250.063.8028834
Produced by FastQC (version 0.11.5)