FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S3_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S3_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981723
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA12540.12773460538257736No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG12470.12702157329511482No Hit
ATGTTATGCAGTGCAGTTTTGGTGTAAAATAAACATTGAGGTATAAATCA11540.11754843270454088No Hit
GAGCAAAAGCAGGGCGGCTGGGGGGAGGCGCAGAGACTGAGCTGCTCAGT10170.10359337613563094No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG10030.10216731196070583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAGC24250.068.463914
GAGCAAA24600.067.784551
GAGCGAA4100.067.195121
GCAAAAG24950.066.8336643
AGCAAAA31400.053.5668752
AGCGAAA6400.049.843751
CGAAAGC7100.046.9718323
GCGAAAG7350.045.374152
AGCAGGC18700.041.0962568
GTTATGC6200.040.927423
AAGCAGG48150.040.804787
GCAGGCC10950.040.3881269
CAGTACG1651.366061E-939.5454569
AAAGCAG50850.038.3536
AAAAGCA48700.034.5379875
AATATCG850.002741961534.1176457
TAACCGT850.002741961534.1176457
CAATCCG850.002741961534.117645145
ATGTTAT8500.032.4117621
GACGGCT900.003628199532.222227
Produced by FastQC (version 0.11.5)