FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S20_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S20_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114537
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG129601.162814693455668No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA105490.9464916821962842No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA83830.7521508931511471No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA69290.6216931335612905No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT52240.468714811621328No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA50470.4528337776134843No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC43000.38581043069902565No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG41890.37585113818563226No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT38370.3442685168818981No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT34610.3105325350347274No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC34100.305956643879925No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA33320.2989582221137566No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC30860.2768862765435333No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG28470.2554423944651456No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT27080.24297084798440968No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA26650.23911274367741944No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT26630.2389332969654664No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA26190.2349854693024996No Hit
GTAAAGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT25280.2268206439086365No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG24310.21811747837891432No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC23870.2141696507159475No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA23650.21219573688446414No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC23490.21076016318883986No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG22960.20600482532208442No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA21300.19111074822998247No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC20660.18536845344748537No Hit
GTGCCTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC20580.18465066659967322No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20500.1839328797518611No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA19910.17863920174924655No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC19300.17316607703467898No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT18730.16805184574401746No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC18640.1672443355402288No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA18160.16293761445335597No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG17970.16123287068980213No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG17940.16096370062187257No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG17930.16087397726589606No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG17270.1549522357714459No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG16900.15163247160031476No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA16890.15154274824433822No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA16600.1489407709210192No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG16380.14696685708953583No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG15860.14230124257875693No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC15240.13673839450821282No Hit
GTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT15180.13620005437235372No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC15040.13494392738868247No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG14780.13261112013329301No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA14770.1325213967773165No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA13890.12462574145138294No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC13780.12363878453564126No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA13690.12283127433185259No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT13630.12229293419599349No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA13620.12220321084001698No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT13610.12211348748404045No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG13600.12202376412806394No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA13100.11753759632923805No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT12960.11628146934556682No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA12960.11628146934556682No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT12680.11376921537822433No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT12600.1130514285304122No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA12540.11251308839455307No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT12530.11242336503857656No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA12380.11107751469892879No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT12250.10991111107123407No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA12040.10802692059572719No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG11790.10578383669631426No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA11730.10524549656045516No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA11720.10515577320447864No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA11550.10363047615287782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG100.007091252144.99808145
CGGTGGA151.230108E-4144.998067
GAGCGAA3250.0138.35551
GCAAAAG34750.089.321833
AGTAGAA9850.083.201341
GTAATTT3900.081.793785145
AGCGAAA7750.081.415241
AGCAGGC4150.080.3603748
CAAAAGC41550.074.7035754
CGTACCT406.6535984E-572.521815
ACGGCCG502.3065968E-672.49904145
GGACGCC300.001935902872.49903145
GTAAAGC5450.071.85971
GAGCAAA43750.071.281781
TAAACTA27350.068.146645
TTTAAAC28200.066.606913
AAGCAGG53750.065.822387
GCAGGTC26800.065.736079
GCAGGGG6050.064.7098859
CTATTCG29700.062.978969
Produced by FastQC (version 0.11.5)