FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S17_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S17_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114694
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT44220.3967007986048189No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC38240.34305378875278775No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA37190.33363416327709666No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA36960.33157081674432626No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT36710.32932804877392363No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA34060.30555470828765563No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG32460.2912009932770787No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA29390.26365980260053434No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT25240.2264298542918505No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG23440.2102819249049515No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23210.20821857837218108No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA22090.19817097786477725No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA20900.18749540232566067No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG19830.1778963554123374No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA19540.17529474456667032No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG18690.16766933346730134No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG18030.16174842602543837No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT17920.1607616081184612No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT17860.16022334380556458No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA17830.15995421164911627No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT17790.15959536877385186No Hit
GTTGCAACAGCATCATACTCCATGTTTTTGGCTGGACCATGTGCTGGCAT17230.15457156852014992No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC16370.14685644670196485No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG16100.14443425729393No Hit
GGAAAATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGG15790.14165322501063074No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG15530.13932074632141198No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT15260.13689855691337713No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA15230.1366294247569288No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA15000.1345660782241584No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT14980.13438665678652617No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT14830.1330409960042846No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC14550.13052909587743364No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT14530.13034967443980142No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA13920.12487732059201896No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA13710.12299339549688075No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA13670.12263455262161634No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT13550.12155802399582308No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT13420.1203917846512137No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT13420.1203917846512137No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA13410.12030207393239759No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA13310.11940496674423652No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT13180.11823872739962717No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG12830.11509885224106346No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA12800.11482972008461514No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA12290.11025447342499375No Hit
GTTGCATACTCCATGTTTTTGGCTGGACCATGTGCTGGCATCATCACTGC12240.10980591983091323No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA12160.1090882340803844No Hit
GTATGCAACAACACACTCCTGGATCCCCAAAAGAAATCGATCCATCTTGA11860.10639691251590122No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC11750.10541009460892407No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA11460.10280848376325699No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT11350.10182166585627984No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG11310.10146282298101543No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA11160.10011716219877384No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT11150.10002745147995773No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT11150.10002745147995773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA100.007087291145.025181
AGCGTAT100.0070910943144.999159
GAGCGAA2950.0115.528531
AGACGTA203.864195E-4108.7643
CAGACGT203.864195E-4108.7642
AGCAGGC8000.099.686928
GCAAAAG30000.094.020443
TTAGCAG259.3822327E-487.011213
GCAGGGG7050.077.1272059
TAAACTA5050.076.098915
CAAAAGC37550.075.3092964
AAGCAGG42950.073.934377
GCGCAAA406.657793E-572.512591
TGTGTAG406.659274E-572.509332
AGCGAAA4850.071.761812
CTATTCG5500.069.863229
TTAGTAG651.3965291E-766.934691
GATCTTA651.3965291E-766.934691
TTTAAAC5800.066.258533
ACTAATA554.0620507E-665.908718
Produced by FastQC (version 0.11.5)