FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S14_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S14_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314231
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATGCAGTGCAGTTTTGGTGTAAAATAAACATTGAGGTATAAATCA3900.12411251595164066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG6500.071.384613
GAGCAAA6550.064.198471
GAGCGAA902.0434527E-856.388891
AGCAAAA7700.055.551952
CAAAAGC9350.051.951874
GCGAAAG1351.891749E-1048.3333323
ATTCGTG450.00963303148.3333327
CGAAAGC1402.7102942E-1046.6071434
AAGCAGG12000.045.31257
AGCGAAA1351.02209015E-842.962961
AAAGCAG13300.040.8834576
AGCAGGC6550.035.4198468
CCCTATA1501.1306092E-633.8333322
GCCCTAT1956.9594535E-933.461541
GACAGTC1601.8717037E-631.7187487
TATAGGG1153.1572027E-431.521742
GTTATGC1703.0030587E-629.8529423
AAAAGCA16850.029.258165
ATACTAC1753.7637874E-629.06
AGCAGGG8250.028.121218
Produced by FastQC (version 0.11.5)