FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111143
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA114311.0287604745743797No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG101880.9168936851512361No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA80070.7206093185125587No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA54210.48787599795885855No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA49860.448727121531612No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40680.3661094926575607No Hit
GTAAAGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT36710.3303805180791311No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT34370.30932112248378474No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC31650.28484182503962135No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA30740.2766520600858755No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG29120.2620724785198665No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG27320.2458729434465231No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT27170.24452298219041113No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG26750.2407430906732977No Hit
GTGCCTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC26410.2376831784927773No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG22970.20672406701927656No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC20920.1882745965190799No Hit
GTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT20280.18251476182633558No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC19850.178644872892148No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG19760.17783489613848083No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG19530.17576495554577584No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT19490.1754049658774793No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC19080.17171507177743997No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG18960.17063510277255042No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT18560.16703520608958522No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG18450.1660452345017698No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT18100.16289532490417524No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA17610.1584854514675429No Hit
GAGAACTTACTATCAGTCTCTGTCCATCCATTAGGATCCCAAATCATCTC17600.15839545405046876No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC17440.15695549537728268No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC17140.15425557286505878No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA17030.15326560127724334No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC16880.1519156400211314No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA16470.14822574592109206No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16420.14777575883572142No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT15560.14003598096734624No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT15560.14003598096734624No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC15300.13769604812341885No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG15240.13715606362097407No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA14970.13472613335997258No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA14800.13319617726971236No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG14660.13193621343067452No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT13740.12365645105985458No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA13540.12185650271837199No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG13380.12041654404518591No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA13240.1191565802061481No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT13140.11825660603540677No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC13140.11825660603540677No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA13020.11717663703051723No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG12750.1147467067695157No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT12730.11456671193536745No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG12660.11393673001584853No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG12470.11222677909144008No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA12460.11213678167436596No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC12350.11114681008655052No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC12210.1098868462475127No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA12180.1096168539962903No Hit
GGATAATGCAGTGATGATGCCAGCACATGGTCCAGCCAAAAACATGGAGT12150.10934686174506791No Hit
GTGCTTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC12070.10862688240847489No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11990.10790690307188183No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA11960.10763691082065945No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT11900.10709692631821466No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA11760.10583696247917684No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA11700.10529697797673207No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT11630.10466699605721316No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA11580.1042170089718425No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA11410.1026870528815823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2800.0126.885951
ACGGCCG501.9463187E-10101.499626145
GCAAAAG35350.093.1197743
GTAAAGC6750.089.155841
CTGGCCG259.387163E-486.99968145
AGCAGGC2550.085.29388
GCAGGGG9800.080.637469
AGCAAAA40750.080.424122
TAAAGCA7500.079.2735062
AGCGAAA6650.078.503011
CAAAAGC43550.076.252274
GAGCAAA44450.073.892771
TAAACTA23550.072.6601945
CTATTCG24250.070.855419
GCAGGTC28050.070.3027659
TTTAAAC24400.069.831853
AAGCAGG53700.068.179457
TTTTTAA25100.067.8843461
TTTTAAA26050.065.4087142
TTAGTAG1250.063.8055041
Produced by FastQC (version 0.11.5)