FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B2S11_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B2S11_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132732
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG99180.8755822206841513No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA98270.8675485463463556No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA67270.5938739260478207No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA55960.4940268307066455No Hit
GTAAAGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT54780.4836095387081851No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT48170.4252550470896911No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA47260.4172213727518954No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40800.36019111316710395No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG34550.3050147784294961No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC32660.2883294548048435No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG31760.280384062602628No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG27060.2388914588799469No Hit
GTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT27050.23880317674436674No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC26650.2352718913211598No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA26210.23138747735563223No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT25980.22935698823728828No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA25070.22132331389949256No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG24920.21999908186578995No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT24470.2160263857646822No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT24180.2134662038328572No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC24140.2131130752905365No Hit
GTGCCTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC23590.208257557833627No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA22970.20278406542765634No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC22600.19951762641118995No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT21330.18830579519250804No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20660.18239089210863646No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT20070.17718224610940628No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT19990.1764759890247649No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG19850.1752400391266425No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG19570.17276813933039767No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA19560.1726798571948175No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA18820.1661469791618847No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT18630.16446961858586145No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC18120.15996722967127264No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG18010.15899612617989073No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC17800.15714220133270712No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC17410.1536991980450804No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA17360.15325778736717954No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC17240.15219840174021745No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA16750.147872577096789No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16670.14716632001214763No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG16650.14698975574098727No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC16580.14637178079192606No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA16520.14584208797844503No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT15840.1398389027589933No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG15650.13816154218297003No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA15420.13613105306462606No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC15290.1349833853020838No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT15150.1337474354039614No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA14930.1318052284211976No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA14660.12942161076053293No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG14580.12871535367589157No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA14280.1260668896084864No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG13850.12227075777853896No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA13810.12191762923621828No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT13730.12121137215157689No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG13610.12015198652461483No Hit
TCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACT13390.11820977954185104No Hit
CTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTATTGTCT13230.11679726537256827No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT13210.11662070110140793No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT13050.11520818693212516No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC12800.11300113354262085No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA12710.11220659432239931No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT12710.11220659432239931No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG12540.11070579801753637No Hit
GAAAAAAGGTCCTGCATTCCAAGTGAGAACATGAAATAAAGGGCTCTCTT12510.11044095161079584No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT12480.11017610520405532No Hit
GGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGG12400.10946984811941395No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA12350.10902843744151308No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT12320.10876359103477255No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC12220.10788076967897084No Hit
TTCCAAGTGAGAACATGAAATAAAGGGCTCTCTTATGACAAAAACGTCTC11960.10558543415388635No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA11920.10523230561156566No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA11740.10364322717112257No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA11610.10249555940858032No Hit
GTGCTTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC11380.10046507029023634No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA11350.10020022388349582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG100.007084676145.043181
GACGTAC100.0070856116145.036774
GAGCGAA2950.0140.126461
CTAGTAG256.4570995E-6116.029423
TAGTAGT256.4570995E-6116.029424
GTAATTT3750.094.73227145
AGCAGGC3900.092.947678
GCAAAAG35700.091.612873
TCTAGTA353.433152E-582.878172
GTAAAGC11250.081.868821
CAAAAGC40450.080.675584
GTTCGCC451.2330747E-680.55465145
GCAGGGG12600.079.9792569
TTAGTAG1200.078.561592
ATAGGAC755.638867E-1177.352943
AGCGAAA6650.076.338521
GAGCAAA43250.075.959031
TAAAGCA12250.075.182332
AGCAAAA43700.074.841642
GTCTAGT502.3023604E-672.521591
Produced by FastQC (version 0.11.5)