FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B1S6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B1S6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205295
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG76050.6309658631289435No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT58970.48925781655113476No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG47270.39218614530052803No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA35620.29552931025184703No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT35040.29071721030950926No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC34540.2865688482902526No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA31930.26491439854973264No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA28140.23346981444376688No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT28110.23322091272261147No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG26800.22235220423215893No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA26590.2206098921840711No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA24840.20609062511667267No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT23600.19580268730891606No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT23500.19497301490506472No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA23060.19132245632811884No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC22350.18543178226077434No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT22190.1841043064146122No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG21980.1823619943665244No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC21720.18020484611651091No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG19390.16087347910677469No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA19240.1596289705009977No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA17820.14784762236630866No Hit
CTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTATTGTCT17220.14286958794320062No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT17200.14270365346243036No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA17060.14154211209703849No Hit
GGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGG17030.14129321037588308No Hit
TCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACT17000.1410443086547277No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA16840.13971683280856553No Hit
GAAAAAAGGTCCTGCATTCCAAGTGAGAACATGAAATAAAGGGCTCTCTT16750.13897012764509933No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG16690.13847232420278854No Hit
TTCCAAGTGAGAACATGAAATAAAGGGCTCTCTTATGACAAAAACGTCTC16560.1373937500777818No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA16300.1352366018277683No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT16280.13507066734699805No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG15650.1298437312027346No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG15540.12893109155849813No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT15450.12818438639503194No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG15040.12478272953924142No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT15000.1244508605777009No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA14890.12353822093346442No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA14560.120800302000755No Hit
GTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT14270.11839425202958612No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA14220.11797941582766046No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA14170.11756457962573477No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT13920.11549039861610644No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT13880.1151585296545659No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT13690.11358215208724835No Hit
ATAACAACCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAAT13180.10935082282760651No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG13140.10901895386606598No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA13120.10885301938529572No Hit
AATTAGCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCC12870.10677883837566736No Hit
TTTGAATGCTTGTCATTCAGTAAGGCACCTTGGGTCAGAAAAAAGGTCCT12820.1063640021737417No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC12390.10279641083718095No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA12300.10204970567371473No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA12130.10063926258716746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTGA100.0070885248145.017214
AAGTACG100.007091163144.999168
ACGGCCG403.274181E-11126.874275145
GAGCGAA2750.0126.570981
GCAAAAG24200.096.47843
AGCAGGC5300.095.7541668
CAAAAGC30200.077.5506064
GCAGGGT3100.077.176979
GCAGGGG15100.074.900249
AGCGAAA5750.074.406311
TAGTAGT406.659823E-572.5086062
AAGCAGG38050.070.30847
TAAACTA2100.065.603035
AGCAGGG20900.065.561838
TCTATGC2350.064.794933
GAATAAG902.8194336E-1064.457451
AACAGAC4250.063.1172877
TGTACCC350.003565003462.1502383
ACAGACT4400.059.317848
GTAGTAG502.0084069E-458.01171
Produced by FastQC (version 0.11.5)