FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B1S4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B1S4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852932
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA8830.10352525171994954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA100.0070882556145.016591
GAGCAAA18850.057.3142551
GCAAAAG19750.056.167693
AGCAAAA20450.052.8269122
GCGAAAG4450.050.5084342
CGAAAGC4650.048.336033
AGCGAAA4600.045.7117541
GAGCGAA3250.044.620491
CAAAAGC25900.043.950334
CGGACTC700.001056263641.430886
CAGTACG1053.8235303E-641.4284559
AAGCAGG38950.033.1321267
AGCAGGC15450.032.3785488
AAAGCAG40200.032.2841386
CGATACT900.003626943632.224024
GCAGGCC8850.031.9490629
CCGACTA950.004726974830.526239
AAAAGCA42150.028.038345
TAATACG1358.079099E-426.853354
GCCCTAT4101.8189894E-1224.7589321
Produced by FastQC (version 0.11.5)