FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_B1S11_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_B1S11_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194856
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG87610.7332264306326453No Hit
GTAAAGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT62580.5237451207509524No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA62070.5194768239854844No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA58380.488594441505922No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA43620.3650649115876725No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT36510.30555983315144253No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC34790.2911647930796682No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT34560.28923987493053555No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG34190.28614326747323526No Hit
GTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT31730.2655550124868603No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT31620.2646343994590143No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG31570.26421593899181156No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA31190.2610356394410707No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC30930.25885964501161646No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC30530.25551196127399456No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA30460.25492611661991066No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG30160.2524153538166942No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC26770.22404373414034828No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT23880.1998567191360298No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA22700.18998105211004507No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT22310.18671706046586367No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT21200.1774272380939628No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT20750.1736610938891381No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC20030.1676352631614186No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA20010.16746787897453752No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA19250.16110727987305584No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA19200.16068881940585308No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA19150.16027035893865033No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA18850.1577595961354339No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG18560.15533252542565798No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA18230.15257068634211987No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG18050.15106422866019No Hit
GTGCCTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC18030.15089684447330892No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA17670.14788392910944917No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT17350.14520578211935162No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA17090.14302978768989735No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA16960.14194179047517022No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT16940.14177440628828913No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT16900.14143963791452696No Hit
TCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACT16620.13909625929819158No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG16410.13733872533594008No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG16330.13666918858841567No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG16250.13599965184089127No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC15850.13265196810326935No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT15370.12863474761812302No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA15370.12863474761812302No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC15300.12804890296403917No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC15190.12712828993619313No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT15160.1268772136558715No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA14760.12352952991824956No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG14710.12311106945104681No Hit
CATCAATACTGCCTTGCTTAATGCATCTTGTGCAGCAATGGATGATTTCC14690.12294368526416571No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA14680.12285999317072518No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA14500.1213535354887953No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA14350.12009815408718708No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA14340.12001446199374653No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC14340.12001446199374653No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG14120.11817323593805447No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG13730.11490924429387306No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC13640.11415601545290814No Hit
ATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGAACCCA13540.11331909451850264No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT13370.11189632893001333No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA13340.11164525264969168No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAAGCACCTTGGG13320.11147786846281058No Hit
ATATGATAGTGATGAACCAGAATTGAGGTCGCTAGCAAGTTGGATTCAGA13260.11097571590216729No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT12960.10846495309895082No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG12860.10762803216454535No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA12820.10729326379078316No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA12760.10679111123013986No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT12710.10637265076293713No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA12700.10628895866949659No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT12640.10578680610885328No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG12600.1054520377350911No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA12590.10536834564165054No Hit
TTTCCAGATAGTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATG12530.10486619308100725No Hit
CTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTATTGTCT12470.10436404052036395No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT12350.10335973539907738No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA12280.10277389074499353No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG12160.10176958562370696No Hit
GGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGG11950.10001205166145545No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAG100.0070893336145.011632
GAGCGAA2600.0117.124791
TACCGGA256.462815E-6116.00932
GTAATTT4200.098.39251145
GCAAAAG28250.097.273293
AGCAGGC3550.095.985598
ATTAGTA651.6370905E-1189.237931
AGTAGAA8550.083.106081
CAAAAGC33650.081.8787844
GCAGGGT3450.079.85489
GCAGGGG10250.077.804619
GTAAAGC11500.076.2887341
AGCGAAA5500.075.1423951
TTAGTAG809.822543E-1172.505812
AAGCAGG43550.069.919397
TAAAGCA12800.069.673562
AGCAAAA40450.069.189732
GCAGGTC16650.068.363129
TCGTACT554.0601535E-665.9143752
TAAACTA21550.065.272065
Produced by FastQC (version 0.11.5)