FastQCFastQC Report
Sun 20 Mar 2016
H5HGTAFXX_n01_sv117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HGTAFXX_n01_sv117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26996029
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC14015735.191774686565939TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1638450.043.7103816
ACACGTC1649550.043.61675313
ACGTCTG1644000.043.5915615
TCTCGTA1592850.043.5215241
CTCGTAT1595850.043.4862342
TCGTATG1620900.043.48462343
AATCTCG1589650.043.48457739
CGTATGC1625350.043.441744
ATCTCGT1597600.043.3357940
CACGTCT1655850.043.32715214
ATCGGAA1649650.043.2875752
GCACACG1667250.043.27848411
GTATGCC1634900.043.20866845
TCGGAAG1652100.043.19753
CACACGT1666350.043.18916312
GATCGGA1602500.043.151461
CGGAAGA1677450.043.0167544
ACCAATC1604050.042.5766836
GACCAAT1629900.042.4880835
ACTGACC1636100.042.42977532