FastQCFastQC Report
Sun 20 Mar 2016
H5HGTAFXX_n01_sv116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HGTAFXX_n01_sv116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26954564
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC7245402.6880048959426683TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAT835800.043.67730331
TCGTATG825500.043.67187543
CGATGTA825300.043.66296834
CTCGTAT824100.043.65870342
ACCGATG833600.043.6493632
TCTCGTA830700.043.60434341
CGTATGC830900.043.58580844
CCGATGT828450.043.52139333
TATCTCG826700.043.45599439
GTCACCG846800.043.4190329
CGTCTGA861200.043.2535416
ATCTCGT839300.043.2003640
TCACCGA850300.043.16886530
ACGTCTG865500.043.03864715
ACACGTC870550.042.95180513
GTATGCC847600.042.7934145
CACGTCT877200.042.51333214
ATCGGAA876450.042.2897382
GCACACG886000.042.2571911
GATCGGA852050.042.1544461