FastQCFastQC Report
Sun 20 Mar 2016
H5HGTAFXX_n01_mb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HGTAFXX_n01_mb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38856063
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC388626510.001695230934745TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4242350.044.4710351
ATCGGAA4415950.044.4517022
TCGGAAG4413850.044.3887063
CGGAAGA4480300.044.1832964
ACACGTC4481350.044.10115413
CGTCTGA4465000.044.05811716
ACGTCTG4468650.044.0326515
GCACACG4504150.043.9775811
CACGTCT4481850.043.95868314
CACACGT4504800.043.90354512
AATCTCG4366850.043.80827339
GTATGCC4428250.043.7867345
TCTCGTA4389900.043.76218841
CTCGTAT4383350.043.76188342
TCGTATG4423300.043.7242243
CGTATGC4434150.043.70247744
ATCTCGT4393000.043.70052340
GTAATCT4369450.043.3475237
GAACTCC4538350.043.25007221
GAAGAGC4598700.043.1945956
Produced by FastQC (version 0.11.2)