FastQCFastQC Report
Sun 20 Mar 2016
H5HGTAFXX_n01_mb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HGTAFXX_n01_mb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46790342
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC646159613.809678929040528TruSeq Adapter, Index 7 (100% over 51bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC653130.13958649842738916TruSeq Adapter, Index 7 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7108900.044.6419071
ATCGGAA7346350.044.6334082
TCGGAAG7347750.044.5624733
CGGAAGA7437700.044.4978874
ACACGTC7453300.044.45625713
CGTCTGA7428100.044.4078216
ACGTCTG7431250.044.40711615
CACGTCT7446750.044.35970314
GCACACG7485150.044.3396311
CACACGT7478800.044.31009312
GTATGCC7230000.044.21186445
TCTCGTA7218200.044.06678841
CTCGTAT7176450.044.06481642
CATCTCG7216300.044.0530939
TCGTATG7196350.044.04738643
CGTATGC7249350.044.02805744
ATCTCGT7235950.043.99217640
GAACTCC7500400.043.86427721
GAAGAGC7584650.043.8198856
GAGCACA7591000.043.792789
Produced by FastQC (version 0.11.2)