FastQCFastQC Report
Sun 20 Mar 2016
H5HGTAFXX_n01_bgm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HGTAFXX_n01_bgm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31142253
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT32850.014.58858415
AATCCGC13900.014.56812326
CGAACTC33400.014.21362716
CTCGAAC33000.014.11319414
ATCCGCC16750.012.89532627
AGACCCG17650.011.7330851
CGATTCG6100.011.4360336
GCCTCGA37950.011.14591312
CCTCGAA40100.011.05326913
CGGTCCG5300.011.03721336
AGACGCG4701.8189894E-1210.536441
AGACCAG205900.010.5278341
AGACCGA14050.010.4137251
GGCCTCG39500.010.252645511
AAATCCG21950.09.94290225
GATTTCG172150.09.8693454
TCCTCGC158600.09.84519918
CCTCGCC155650.09.77138319
TTCCTCG171900.09.62010317
ATTTCGT177300.09.5192115
Produced by FastQC (version 0.11.2)