FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8777944
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC2041172.325339510026494No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA2034122.317308016546927No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC1512511.7230800287629997No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA1342771.5297090070294366No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1268511.4451106090446693No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1189431.3550211758015316No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA1026851.169806961630195No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1010151.150782005444555No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC862210.9822459564563183No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT812190.9252622254140604No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT809820.9225622765422061No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA771380.8787707007472365No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC636980.7256596761155004No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG545850.6218426547264371No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA495180.5641184313775527No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC471250.5368569223043574No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT440310.5016094885089264No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT435920.49660831739186306No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG410000.4670797626414568No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC396180.4513357569836399No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG395870.45098259911432564No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG379850.4327323118033107No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC320140.3647095492976487No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC300760.34263148637084034No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC287380.32738873704366306No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG260850.297165258743961No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG260530.296800708685314No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG255800.2914122031309382No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA251250.28622875698455125No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA244020.2779922040969959No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC218150.24852061029325317No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA213820.2435877923121861No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC207030.23585249575527023No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT183370.20889857579405838No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC181650.2069391192288308No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA180590.20573154715956263No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC175820.20029747284785593No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT173630.1978025833839906No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG172340.19633299096006993No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT162870.18554458766198553No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT148750.16945881632418708No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT144990.16517535313508494No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT142740.1626121105352233No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG138810.1581349801274649No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT138650.1579527050981414No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT134340.15304267149573977No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG133740.15235914013577667No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT131010.14924907244794453No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT127620.14538712026415296No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG125420.1428808386109549No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA122070.13906445518449423No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA120410.13717335175526296No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAAACAAGACGGGT120190.13692272358994315No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT114470.13040639129162818No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT109880.12517737638791043No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT107330.12227236810806721No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT106310.12111036479612995No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT103880.11834206278827934No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT103080.11743068764166187No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA103070.11741929545232915No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT98890.11265736031125283No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG97940.11157510232464458No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG93970.10705240315955536No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC93580.10660810777557933No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC92160.10499041689033331No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT90080.10262084150912788No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC89260.10168668198384496No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT87880.10011455985592982No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)