FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_lncHEK_INP_R1__lncHEK_INP_R2__lncHEK_D7_R1_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_lncHEK_INP_R1__lncHEK_INP_R2__lncHEK_D7_R1_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31395651
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA23797527.57987786270143No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA23372727.444572498273725No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC20262046.453772848984721No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC16489905.2522879681647625No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT15146494.82439112347121No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA15025494.7858507536601165No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA13226554.212860564668654No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT12607454.015667647726113No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT11417873.636768035165125No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT10719333.4142722506375165No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC6793212.1637423603670456No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT6203231.975824613415406No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC5956471.8972277402370157No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT5816951.852788464236655No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA4236851.3495021969762626No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC3983081.2686725304724529No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC3932431.2525397227788015No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA3829451.2197390014304847No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC3680191.1721973849180576No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC3014130.960046982303377No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA3004720.9570497518907953No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC2940430.9365723934184387No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA2634620.8391671827413294No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC2615590.8331058336710393No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT2498990.7959669318530773No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA2110200.6721313088873361No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT2059910.6561131667567588No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT1914100.6096704285571272No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC1897420.6043575908013502No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1887560.6012170284349255No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC1863690.5936140645721919No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA1804080.5746273584198015No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1774050.5650623393666848No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1763460.5616892607195818No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA1758380.5600712022184219No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT1664790.5302613409736272No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA1615170.5144566041965494No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT1587020.5054903941950432No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT1586990.5054808387314537No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT1556530.49577885803355376No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT1410490.4492628612797358No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT1400080.44594711541417No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT1359020.43286887091463716No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1347620.4292377947506169No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA1254730.3996508943229111No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT1238890.3946056095476409No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC1203470.38332379220293916No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC1182640.37668911531727756No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT1162630.37031562110306293No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1048350.33391567513602444No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1006490.3205826182741042No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT946240.30139206223180404No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA935050.2978278743129104No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT895180.2851286632024289No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA876470.27916923907709384No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA857770.27321300010628863No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC794640.25310511955939374No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC761880.24267055331963017No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT748820.23851074150365603No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC685380.21830412116633607No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC613200.1953136757699339No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC584170.18606717216980148No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC534750.1703261384833205No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT518870.16526811308993083No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA507720.16171666578915658No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT452750.1442078713386131No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGTACCCCTACCAAC428700.13654757469434223No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC392780.12510649962314843No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT353260.11251876892121142No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC345200.10995153437015846No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT340020.10830162432370014No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAA333110.10610068254357904No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC318100.10131976559428567No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT317010.10097258375053282No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC314490.10016992480901257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)