FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_lncHEK_D7_R2__lncHEK_D14_R1__lncHEK_D14_R2_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_lncHEK_D7_R2__lncHEK_D14_R1__lncHEK_D14_R2_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34262980
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC27619888.061143543264478No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA27362457.986009973446559No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC23324006.807347171787159No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA17820975.201231766764012No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT14811724.322951477075257No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT12525203.655607305610895No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA12148053.5455322333317185No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT11213023.2726341958580365No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT10387833.0317940821259564No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC9801102.8605509503259787No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA9075782.6488589141983563No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC8200612.3934316279553034No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT6076371.773450528821486No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC5917191.7269922230932626No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC5719111.6691805558068797No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT5638351.6456099265154405No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA5438401.5872524806657216No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC4999831.4592513552528121No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA4964341.4488932369572056No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC4240081.2375105726355384No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC3893911.1364773291756876No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC3716361.0846575516782253No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA3426461.000047281351476No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC2826710.8250041298217494No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT2643010.7713894121293595No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA2598210.7583140754248463No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC2546280.7431577755349943No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA2418240.7057879962571849No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC2325750.6787938468866397No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT2215250.6465432954168026No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA2188020.6385959423260907No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT2024730.5909380911993061No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1863990.5440244835679792No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1820990.5314744952132009No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA1750690.51095672355411No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC1722060.502600766191382No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC1617570.47210429448927094No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT1610640.47008170334279153No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT1579150.4608910258243737No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT1566970.45733616865783416No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA1488450.4344193062016205No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT1478890.4316291227441396No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1360800.397163352399587No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT1357720.3962644230011517No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT1328420.38771291930824464No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1325760.38693657119141417No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT1313840.38345759767539195No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT1305620.3810585068782692No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT1220860.3563204368096412No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC1133890.3309373557116164No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA1053390.30744261007069434No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC1033240.3015616271556064No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC1022000.29828111857170625No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1003230.2928029027247484No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA966990.282225889283419No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT898860.2623414542459529No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT839420.2449932842969292No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC816800.23839140670192724No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC789920.23054620467921938No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA785680.22930871745539938No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT718440.20968403799085777No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC709920.2071973891354459No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA678100.19791039775291No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGTACCCCTACCAAC606600.17704239386066245No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC554770.1619152799902402No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT488310.1425182514772504No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA479250.13987399811691803No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA472380.13786891858209646No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC469300.1369699891836612No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC461290.13463218902734087No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA454720.13271466755080846No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGTACCCCTACCAA450340.13143631989978688No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT439040.12813829970422888No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC416170.12146345706065262No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC396590.11574883445631408No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAA369710.10790363243360619No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC369010.10769933029759816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)