FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_lncHAP_INP_R1__lncHAP_INP_R2__lncHAP_D7_R1_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_lncHAP_INP_R1__lncHAP_INP_R2__lncHAP_D7_R1_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23559792
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA17405537.38781140342835No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC16158096.858333044705997No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC12987295.512480755347925No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT12104435.137749093879946No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA11316974.803510149834939No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT10295934.370127715898341No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT9086663.8568506886648235No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT8517183.615133783863626No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA7932663.367033121514825No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA5904192.5060450448798526No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT5609702.3810481858243913No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC5521252.3435054095553984No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT4977152.1125610956157845No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC4806732.0402259918084167No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA3475181.4750469783434421No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC3206991.3612132059569966No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC3172691.346654503571169No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA3007641.2765987068137106No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC2889981.2266576886587115No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC2348710.9969145737789197No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC2323650.9862778075460089No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC2120240.899940033426441No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA2065120.8765442411376128No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT1987760.8437086371560496No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1838100.7801851561338063No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT1795960.7622987503455039No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT1780650.7558003907674568No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1712110.7267084531136778No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA1616590.6861648014549534No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT1593530.6763769391512454No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA1581980.6714745189600995No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC1548110.6570983309190506No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT1531040.649852935883305No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC1452750.6166225915746625No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT1386700.588587539312741No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT1386330.5884304920858384No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1371990.5823438509134545No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA1359630.5770976246309815No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT1286170.5459173833113637No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT1264850.5368680674260622No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT1170540.4968380026445055No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT1111280.4716849792222274No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1104900.46897697568807056No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT1101270.46743621505656757No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA1079090.4580218704817088No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT1066990.4528860017100321No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT1028140.43639604288526823No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1012560.4297830812767787No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC967660.4107251880661765No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC942300.3999610862438854No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA921450.3911112627819464No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC774360.3286786233087287No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT732440.3108855969526386No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA636500.2701636754687817No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC627540.2663605858659533No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA622000.2640091219820616No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC608720.2583723998921552No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAAACAAGACGGGT581220.24669997086561715No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC559860.23763367690173157No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT559380.2374299399587229No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA502750.21339322520334647No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC482020.20459433597715973No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC475230.2017123071375163No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAAACAAGACGGGT458600.1946536709661953No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA430760.1828369282716927No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC416280.17669086382426466No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT401520.17042595282674822No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT387980.16467887322604546No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT384900.16337156117507318No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT363500.15428828913260356No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT355800.15102000900517287No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGTACCCCTACCAAC345900.14681793455561917No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC313800.1331930264919147No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA312910.13281526424341947No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT300320.127471414009088No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT291960.12392299558502044No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA285350.12111736809900528No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGTACCCCTACCAA284990.1209645653917488No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA278850.11835843032909628No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC265870.1128490438285703No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT264820.11240336926573885No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT263900.11201287345830559No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC252820.10730994569052223No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT252490.10716987654220377No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC242780.10304844796592433No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT236220.10026404307813923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)