FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_eRNA_lib02_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_eRNA_lib02_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505693
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT67358026.881984345249No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG1317665.258664968134564No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT995063.9711967906682903No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG965053.8514295246863837No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT795443.17453095810221No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG706352.8189806173381973No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG675462.6957013488883117No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT629192.5110418554866856No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT600462.3963829567309323No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT481061.9198680764163847No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT411381.641781335542702No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG367961.4684959410430567No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG365991.460633844609056No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT362031.4448298335031466No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT327891.3085801013931075No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT289301.1545708113483975No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT223570.8922481724616703No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG213390.8516206893661754No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT205250.8191346665373611No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT204800.8173387561844169No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG198690.7929542845033289No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT194310.7754740904013381No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT181170.7230335080953653No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG170270.6795325684351594No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT167530.6685974698416766No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG164050.6547090964455741No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG154650.617194524628516No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG133270.5318688283041857No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT128930.514548270678012No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCAACTGG120380.4804259739720708No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT115670.4616287789445873No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT113180.45169140832496235No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG100970.40296237408174107No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG99660.3977342794987255No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT88890.35475215838492585No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT88190.35195852005812367No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGG88130.35171906534439773No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG85140.3397862387770569No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT79110.3157210400476036No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT71760.2863878376161804No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT69810.2786055594200886No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT68420.27305819188543845No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG68080.2717012818409917No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG63640.2539816330252749No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT63220.2523054500291935No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT62040.24759617399258407No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT62030.2475562648736298No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG61400.2450419903795078No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG59020.23554362006838028No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT58670.2341468009049792No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT57090.22784116011019703No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT54400.21710560711148572No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG52120.20800632798990143No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT51100.20393559785656107No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG50530.20166077807616495No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT50060.19978504948531206No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT49340.1969115929206012No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG48790.19471659137811376No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT47250.1885705870591489No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT46690.18633567639770712No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT44700.17839376172579802No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT44400.1771964881571685No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCATCTGG42330.16893530053362485No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT41910.1672591175375435No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTATCAACTGGT41870.16709948106172623No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGTACCCCTACCAACT40850.16302875092838587No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCTACCAACTG39070.1559249277545174No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT38730.15456801771007062No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT37960.1514950155505882No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT37200.14846192251006007No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG36010.14371273735449636No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG35690.14243564554795818No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCTACCAACTG35080.14000118929174485No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG34320.13696809625121673No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAAGTGGT33710.13453363999500337No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGAGTACCCCTACCAACTGG33430.1334161846642825No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT32910.131340910478658No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG32830.13102163752702348No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG31610.12615272501459676No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTGG31070.12399763259106363No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCTACCAACTG30920.12339899580674886No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT30850.12311963197406865No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG29970.11960762950608873No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAAGTGGT29430.11745253708255561No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG28510.11378089813875841No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCAGTACCCCTACCAACTGG28390.1133019887113066No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGG28030.1118652604289512No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT27570.11002944095705261No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCTACCAACTG25750.10276598130736687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)