FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_eRNA_lib01_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_eRNA_lib01_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4382418
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT69907915.95190143888602No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG1996374.555407539855851No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT1696833.871903592948003No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG1468313.3504563006084767No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT1394183.1813031070974973No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT1335353.047062146970006No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1241302.8324545947009163No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG1073432.4494012209697935No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG1032842.3567811194641863No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT1024442.3376136187830556No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT860281.9630258911860987No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC817101.8644958103038096No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT812441.8538624111164204No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG714971.631450947855727No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG714501.6303784805557116No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC677781.5465891204353397No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT648981.4808719752428912No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT618151.4105226840525025No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG568331.2968411502508432No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG562871.2843822748081082No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT493061.125086653075996No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG421690.9622313526459594No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG335730.766083929009054No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG306550.699499682595316No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG289120.6597271186819696No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG262460.5988931224725711No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC259570.5922985895001345No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC256860.5861147886851505No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG251850.5746827436360475No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG240490.5487609808101372No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT223180.5092622383350927No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG220940.5041509048201244No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT215660.4921027615348422No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT215430.4915779371114303No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT212380.48461830888792445No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGTACCCCTACCAACT207800.4741674573260698No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT206870.4720453411792303No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG202300.46161730807056744No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT199500.45522814117685717No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCAACTGG185150.422483660846592No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT184890.42189038106360466No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT183100.41780587794226837No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC178710.4077885769910584No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG178190.4066020174250836No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT177320.404616811997395No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT171190.3906291001908079No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT165070.3766642068374126No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT163200.3723971560905418No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG157200.35870608417544836No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG155550.3549410393987977No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC150750.34398818186672286No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT150710.3438969080539556No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG150370.3431210806454337No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGG135700.30964640981303015No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG132450.3022304125256879No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT130140.2969593498383769No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC128470.2931486681553425No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT124880.2849568434594783No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG119240.27208723585929045No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC118620.27067249176139746No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT115110.2626632146910678No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC107390.24504736882698092No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG103400.23594280600344378No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG97210.2218181834777057No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT96850.2209967191628001No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGTACCCCTACCAAC93880.21421963856482884No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG93030.21228007004352392No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT90040.20545735253916902No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT88930.2029245042348767No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG81420.1857878458878181No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT80170.1829355392388403No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT78300.1786684884919695No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG78300.1786684884919695No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC77630.1771396521281174No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT73970.1687880982599104No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC70960.16191974384917185No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG69590.1587936157618922No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC67270.15349973462138938No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCATCTGG65490.149438049953245No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAACTG65150.14866222254472303No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG64720.14768102905747466No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT59230.13515369825516416No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG59000.13462887383175226No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG57940.13221011779341907No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT56480.12887862362741298No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT55290.12616322769758612No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG54420.12417802226989759No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG54140.12353910558052655No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT53240.12148544479326252No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC51650.11785731073576276No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG50250.11466272728890763No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGAGTACCCCTACCAACTGG48200.10998494438458403No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTGG47240.10779437287816909No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG45640.1041434203674775No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT45370.10352732213129828No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT45090.10288840544192726No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG44690.10197566731425436No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGG44130.10069783393551232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)