FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_circHEK_INP_R1__circHEK_INP_R2__circHEK_D7_R1_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_circHEK_INP_R1__circHEK_INP_R2__circHEK_D7_R1_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12123759
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT229288318.91231094250554No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG8478546.993326079807425No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG6993315.768268735793907No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG6734215.5545561405501385No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG6532305.388015383677621No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG4395913.6258638925435585No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG4286803.535867052454606No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG4091673.3749186205367496No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT3114622.5690217035821976No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG3103862.5601465684034137No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG2307871.9035927718457617No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG2203711.8176788238697257No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT1973381.6276964924822408No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG1866651.539662739914246No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1786861.4738498183607907No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG1565001.2908537690331852No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT1353971.1167905927526274No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1264651.0431170728484456No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG1130130.9321613865798554No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1088760.8980383064361475No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG985730.8130564126192215No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG973150.8026800928655873No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG885440.7303345439314655No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG876090.7226224143848454No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG765830.6316770236029932No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCAACTGG725870.5987169490914492No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG665850.5492108511889753No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT618820.5104192519828215No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGG598920.49400520086220784No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG582720.48064300849266306No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT574240.47364847816588895No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG556850.45930474203586524No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAACTG552370.4556095184670035No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG531400.4383129027886483No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG514160.4240928906620463No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT509650.42037292229250023No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG497410.41027704361328865No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT459170.37873567100764705No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG409560.3378160189426398No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG384840.3174263031787418No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT384610.3172365930401619No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG336660.27768615327968826No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG336330.2774139604721605No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT333090.2747415219982515No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG306920.25315580753461037No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG301780.24891619835069306No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG291990.2408411450607027No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG283580.2339043526021921No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT251470.20741916760305115No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT233180.1923330874524972No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT229120.18898429109321624No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCATCTGG228900.18880282922153105No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGAGTACCCCTACCAACTGG214440.17687583529167808No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG198150.16343940852008026No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG195880.16156705193496507No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTGG193860.15990090202221935No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGG193670.15974418495121853No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG185120.152691916756181No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG184030.1517928556646499No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG178520.1472480606056257No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCAGTACCCCTACCAACTGG175280.14457562213171674No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTG175060.14439416026003155No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG169460.13977513079895434No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG167240.13794401554831304No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT158370.13062780281264252No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT155140.12796361260562833No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT153810.1268665931086225No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG151580.1250272295910864No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTATCAACTGGT142470.11751305844994114No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT131050.10809353765610155No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT122260.10084331105558927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)