FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_circHEK_D7_R2__circHEK_D14_R1__circHEK_D14_R2_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_circHEK_D7_R2__circHEK_D14_R1__circHEK_D14_R2_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9268295
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT171974718.55516036120991No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG6996157.548475744460011No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG5801696.259716592965589No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG4262794.599324902800353No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG3397953.666208293974242No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG2997303.233928138886386No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG2772672.9915642521089367No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT2503402.7010361668462215No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG2187482.360175199429884No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG2169452.340721783240607No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1996752.154387619297832No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG1932982.0855831628147357No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG1921772.073488165838485No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT1693031.826689806485443No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1620641.748584826011688No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1309401.4127733310171935No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT1204731.2998399381979102No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG1169501.2618286319112633No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG1127661.216685485302313No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG896980.9677939685778236No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG797290.860233732309988No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG759040.8189640057853144No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG679300.7329287641362301No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAACTG649970.7012832457318202No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG646500.6975392992993856No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT568910.6138237939124725No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG562750.6071774797845774No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG562640.6070587956037221No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT526560.5681303842831934No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG498950.5383406548885205No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG497940.5372509183188494No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG487950.5264722368029934No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT480730.518682238750493No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG445240.4803904062181879No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG433450.4676696199247003No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT416540.44942462448594916No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCAACTGG378500.4083814768519992No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT346280.37361780133239175No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG326150.35189859623587727No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG316420.34140044096567923No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT312940.33764570506225794No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG310790.33532596880008675No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG305190.3292838650474548No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGG272170.29365703184889996No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT251180.27100993224751696No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG234400.25290519993159477No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT228590.2466365172882391No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG211620.2283267850235669No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT209640.22619046976817206No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG204850.22102231316547433No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG201600.21751573509475042No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG184910.19950810801770985No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG178130.19219284668863043No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG171360.18488837483053788No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT155570.16785180014231313No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT153030.16511127451165505No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT149840.1616694332668522No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTG149650.1614644333181022No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG149120.16089259135579953No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCATCTGG132000.14242101702632468No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTATCAACTGGT122090.1317286512783635No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT121920.13154523027158715No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG121620.1312215461419819No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG120110.12959233602296863No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG118590.12795233643296852No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT118220.12755312600645533No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGACCGTACCCCTACCAACTG115580.12470470566592884No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT109310.11793970735717843No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGAGTACCCCTACCAACTGG108230.1167744444905994No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG107580.11607312887645463No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG104180.11240470874092807No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT102220.1102899724275069No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAACTG98280.10603892085869082No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG97290.1049707632309934No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTGG97200.10487365799211183No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG94140.10157207987013793No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCAGTACCCCTACCAACTGG94140.10157207987013793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)