FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_circHAP_INP_R1__circHAP_INP_R2__circHAP_D7_R1_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_circHAP_INP_R1__circHAP_INP_R2__circHAP_D7_R1_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9622799
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG7829478.13637487388025No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT6976077.249522722027136No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG6849717.118209577067962No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG6403336.65433207115726No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG5668305.89048986682565No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG4110104.271210486678564No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG4019504.177059086446677No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG3774303.9222475705873103No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG2413782.508396985118363No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG2379982.4732720698000654No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG2145002.2290811644304322No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG2122202.2053874345707523No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG1855061.9277758997148335No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1670431.7359086477853274No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG1237001.285488764755452No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1218141.2658894776873133No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1180851.2271377589825996No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAAACAAGACGGGTGGAG1176881.2230121402307166No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG1034121.0746561369514214No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT1030131.0705097342259773No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG938610.9754022712102788No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG929460.9658936033060651No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG796210.8274203794550837No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCAACTGG711980.7398886748024146No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG689460.7164859205725902No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG683220.7100013208215198No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT663950.6899759622953779No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAACTG631970.6567423885711423No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT626990.6515671791544227No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG622360.6467556892750228No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG598580.6220435447108477No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG593930.6172112708578865No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG538430.5595357442257705No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAAACAAGACGGGTGGAG523340.5438542361739032No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGG512370.5324542266756274No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT489920.5091242163532669No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG453700.4714844402340733No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG417500.4338654480884408No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG417420.4337823121941963No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG373950.3886083456590957No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG372540.38714307552303645No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG347000.3606019412854825No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG327120.3399426715657264No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG310390.32255687768184704No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCATCTGG251450.2613065075972178No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG248870.25862537500783295No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT233200.24234113172269317No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG213150.22150519822766745No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG212180.220497175509953No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT211410.21969699252784974No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG210780.21904229736067438No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGAGTACCCCTACCAACTGG198340.2061146658056559No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG190560.19802970009037912No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT189100.19651247002041716No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG188140.19551483928948324No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTGG186460.1937689855103489No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGG173070.1798541152111771No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCAGTACCCCTACCAACTGG172540.17930333991180739No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG172380.1791370681233184No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT168360.17495948943753267No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG167890.1744710660588463No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTG161420.16774745061182303No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT137850.14325353777003969No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAAACAAGACGGGTGGAG135820.1411439644535857No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGAAACAAGACGGGTGGAG127220.13220685582230285No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCATCTGG121300.12605479964821045No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAAACAAGACGGGTGGAG120440.12516108878508217No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAAACAAGACGGGTGGAG120120.12482854520810421No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG119350.12402836222600098No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG119130.12379973851682861No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT118840.12349837090019233No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG112770.11719043492439153No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGACCGTACCCCTACCAACTG107710.11193208961342745No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG107520.11173464186459676No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG103950.10802470258393633No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG98650.10251694959023876No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAAACAAGACGGGTGGAG97960.10179990250238002No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT97040.10084383971856836No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG96540.1003242403795403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)