FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_circHAP_D7_R2__circHAP_D14_R1__circHAP_D14_R2_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_circHAP_D7_R2__circHAP_D14_R1__circHAP_D14_R2_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13028712
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT207474115.92437533349421No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG10102387.753936075952865No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG6908175.302266256250042No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG6121144.6981927300258075No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG5622524.315484139951823No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG5268964.0441142608724485No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG5108163.9206945398747015No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG3440412.640637079091164No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT3100502.3797440606561877No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG2810332.1570282618880516No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG2660872.042312394348728No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG2176471.670518160198798No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT2161641.6591356075719532No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT2099411.6113718685315939No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG2034131.5612671459772847No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG1702231.306522087524845No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1613641.2385261106393326No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG1555701.1940550992300698No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT1527261.1722263873819607No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1420131.0900003008739467No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG1409161.081580435579511No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG1231750.9454119486254665No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG1192810.9155241132047435No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG1058110.81213707080178No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1012370.7770299934483164No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCAACTGG900840.6914267503955879No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG776800.5962216372577734No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG766640.5884234757817964No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG760050.5833654163205081No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT744760.5716297973276253No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT673700.5170887191304866No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGG628840.4826570730859658No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT618000.47433698741671476No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG593470.4555093396799315No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCAACTG592040.45441176380289927No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG586420.4500982138526049No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG564770.43348106858145297No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT532250.40852081157369974No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAACTG531420.40788375704367397No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG517680.3973378181972247No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG513360.3940220644987778No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT445380.34184499588293915No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG432500.33195913763386586No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG421760.3237158055224492No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT416400.3196018148225243No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG405020.3108672599409673No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG366430.28124806197266466No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT329090.25258828347729234No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGAGTACCCCTACCATCTGG327980.2517363189853303No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG319790.24545020259869127No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG311590.23915641085626885No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG304270.2335380504227893No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT302750.23237139634370613No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG299660.22999971140662254No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT271060.20804819386597848No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG267810.2055537032363598No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGAGTACCCCTACCAACTGG253710.19473145158170663No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCAACTGG232130.17816803380103882No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTGG229820.1763950266150637No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCAGTACCCCTACCAACTGG227340.1744915383807701No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG214000.1642526137656585No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGG212260.16291710185933958No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT208950.1603765590950203No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT201700.15481192615202485No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT198700.15250931941699225No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG178040.13665203436840112No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG172980.13276830434197948No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAAACAAGACGGGTGGA171990.1320084441194187No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG166560.12784072592900972No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAAACAAGACGGGTGGA164500.12625960263762068No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCATCTGG160550.12322783710316108No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT157790.12110943890693109No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT152070.11671913539880228No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG148470.11395600731676317No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCAACTGG148230.11377179877796056No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCAGTACCCCTACCAACTG145430.1116226991585968No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGAAACAAGACGGGTGGA144600.1109856446285711No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGAGTACCCCTACCATCTGG144040.11055582470469835No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT138470.10628065153332118No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT136330.1046381253956646No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTATCAACTGGT135470.10397804479828857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)