FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_chr20cas13hap1_R2_FTP_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_chr20cas13hap1_R2_FTP_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663000
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT21504412.93108839446783No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC18475211.109561034275405No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA1582839.517919422730007No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1560629.384365604329526No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1462478.794167167769091No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1401488.42742032471437No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC310111.8647624774503906No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC300691.8081178592904388No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT298391.7942874323511726No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT281931.6953096812988577No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT271681.6336740829825618No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA270981.6294648226097412No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA268851.6166566446181598No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT243151.4621166566446182No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACCAAACACCGTACCCCTACC182711.0986770895971136No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT153280.9217077570655442No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGTACCCCTACC144060.866265784726398No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT141270.8494888755261576No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGAAACACCGTACCCCTACCA139670.8398677089597113No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA132420.7962717979555022No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC125550.7549609140108238No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT110590.665003006614552No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT106770.6420324714371617No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC95370.5734816596512327No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC85330.5131088394467829No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT67500.4058929645219483No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC59220.35610342754058927No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT59090.35532170775706556No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT56340.33878532772098613No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT52600.31629585087191825No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT49830.2996392062537583No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT49720.2989777510523151No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT49140.29549007817197837No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCCTACC42710.25682501503307276No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCGCTAC34330.20643415514131086No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGTAACACCGTACCCCTACCA33920.20396873120865905No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT32330.19440769693325316No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC31420.1889356584485869No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAAGTGGT30270.1820204449789537No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT29100.17498496692723994No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC28920.17390258568851474No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACTAAACACCGTACCCCTACC28080.16885147324113048No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCGCTAC27690.16650631389055923No Hit
GATCGATAACGCATTTTTTGTGGAAAGGACGAAACACCGTACCCCTACCA26630.16013229104028864No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT26460.15911004209260374No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAAGTGGT25180.15141310883944678No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC24470.1471437161755863No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCCCTAC23830.14329524954900782No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC22130.13307276007215876No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACCAAACACCGTACCCCTACCA21950.13199037883343356No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT20240.1217077570655442No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT19800.1190619362597715No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCGCTAC19600.11785929043896572No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGAGTACCCCTACCAAGTGGT17570.10565243535778712No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCGTACC17080.10270595309681298No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC17030.10240529164161154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)