FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_chr20cas13hap1_R2_ETP_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_chr20cas13hap1_R2_ETP_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512728
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT18922012.508527640130943No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC16738711.06524107440333No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1415649.35819261625355No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1331048.798938077433617No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA1306738.638235029694698No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1241848.209274899387067No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC280261.8526793977502893No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC280061.8513572830013065No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA276841.8300712355426751No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT275871.823658979010106No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT272331.8002575479531018No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA259391.7147167236938827No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT244801.6182684527555515No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT227931.5067480736788108No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACCAAACACCGTACCCCTACC165451.0937194260964298No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT149570.9887435150271563No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA149280.9868264486411306No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGTACCCCTACC138130.9131185513853118No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT133290.8811233744599162No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC119350.7889719764557805No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGAAACACCGTACCCCTACCA117660.7778001068268717No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT110200.7284852266897949No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT100670.6654864589007409No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC91810.6069167755207809No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC80340.5310934946665892No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT63830.4219529221380182No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT59840.39557673289580153No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC59140.39094933127435993No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT55260.36530030514408407No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT52270.3455346896467838No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT47900.31664648238149884No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT47750.3156548963197614No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT45950.3037558635789117No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCCTACC40010.2644890555341079No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGTAACACCGTACCCCTACCA35110.23209724418401723No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCGCTAC34250.22641215076338905No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT30940.20453115166771552No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAAGTGGT30670.2027462967565881No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC30430.20115975905780817No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT29100.19236769597706924No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC28880.19091336975318762No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACTAAACACCGTACCCCTACC26790.1770972706263122No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCGCTAC26410.17458525260324395No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT26120.17266818621721816No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAAGTGGT24420.16143021085086015No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC22810.15078718712154465No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCCCTAC22770.150522764171748No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC22580.14926675516021387No Hit
GATCGATAACGCATTTTTTGTGGAAAGGACGAAACACCGTACCCCTACCA21420.14159848961611077No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT19950.13188094621108354No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCGCTAC19610.12963335113781196No Hit
GATCGAAAACGCATTTATTGTGGAAAGGACGTAACACCGTACCCCTACCA18430.12183287411881051No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT18370.12143623969411554No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC17650.11667662659777568No Hit
GATCGAAAACGCATTTATTGTGGAAAGGACGAAACACCGTACCCCTACCA17480.11555282906113987No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACCAAACACCGTACCCCTACCA17240.11396629136235992No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGAGTACCCCTACCAAGTGGT16410.10847951515407925No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCGTACC16120.10656244876805347No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC15210.10054682666017949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)