FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_chr20cas13hap1_R1_FTP_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_chr20cas13hap1_R1_FTP_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134172
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT18928216.689003078898086No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC12376710.9125423657082No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA12124110.689824823748072No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1065959.398486296611095No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC867487.648575348359861No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC653345.7605019344508595No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC204841.8060752690068171No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT204251.800873236158184No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT200511.7678976381007467No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA171561.5126453483245927No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC161461.4235935995598552No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT157271.3866503493297313No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA154171.3593176343623365No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT145931.2866655145780357No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACCAAACACCGTACCCCTACC122931.0838744035296233No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC116491.0270928924360678No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGTACCCCTACC112400.9910313426887634No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGAAACACCGTACCCCTACCA105400.9293123088914202No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC85640.7550882934863495No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT75590.6664773949630215No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC73700.6498132558377389No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT73050.6440822026994142No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA70910.6252138123670836No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT53480.47153341821169986No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC53350.47038720758403485No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT45810.4039069911794683No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCGCTAC39720.35021143177578No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC35110.30956503951781567No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC33260.2932535805856607No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT30860.2720927689980003No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT30750.27112289846689924No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT29650.26142419315588816No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC28360.2500502569275207No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT28040.2472288153824993No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCGCTAC27420.24176227238902037No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCCCTAC27150.23938168108540858No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCCTACC26580.2343559883333392No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT25700.22659702408453036No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCGCTAC23990.21151994582832234No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT23760.2094920347178382No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT23120.20384915162779543No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC20980.18498076129546487No Hit
GATCGATAACGCATTTTTTGTGGAAAGGACGAAACACCGTACCCCTACCA19980.17616375646727303No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGTAACACCGTACCCCTACCA19690.1736068250670974No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCGCTAC19240.16963917289441108No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC19150.16884564245987382No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACTAAACACCGTACCCCTACC18210.16055765792137347No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC17630.1554437951210222No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC16570.14609777000313887No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACCAAACACCGTACCCCTACCA15800.13930867628543114No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT15610.1376334453680747No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC15570.137280765174947No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT15110.13322494295397877No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAAGTGGT14830.13075618160208505No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC14120.12449610817406884No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTGGAAACACCGTACCCCTAC13670.12052845600138251No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCGCTAC12340.10880183957988736No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCGCTAC12200.1075674589039405No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT12160.10721477871081284No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCGTACC11570.10201274586217964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)