FastQCFastQC Report
Tue 19 Oct 2021
H5HCLBGXK_n01_chr20cas13hap1_R1_ETP_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HCLBGXK_n01_chr20cas13hap1_R1_ETP_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305579
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT21498616.466717065761628No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA13418010.277432464829781No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1274029.758275830110625No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1194269.147359141040106No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1095528.391066339149143No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC821636.293223159992617No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT257451.9719220361234364No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC244371.8717366011555028No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC242251.8554985948762963No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT231011.7694065238488057No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA211641.6210432306279436No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA209141.6018946383175587No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT204981.5700313807130784No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT174071.3332781853874793No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGAAACACCGTACCCCTACCA119470.9150729293286733No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGTACCCCTACC112330.8603845496902142No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCCCTAC105050.8046238488823733No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT103430.7922155610652438No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT101090.7742924786627235No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCA97650.747944015643634No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACCAAACACCGTACCCCTACC90030.6895791062815808No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCCTACC84720.6489074962143233No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC76630.5869426514979178No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT70920.5432072666609986No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC66620.5102716878871366No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT63770.4884422926532979No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC48880.37439327685264545No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAGGAAACACCGTACCCGTACC47040.36029991291220215No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT44790.34306617983285576No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCGTACC39790.30476899521208595No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT39580.30316051345801365No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACAGTACCCCTACCAACTGGT38510.29496491594916896No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT37710.28883736640984575No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAACTGGT32900.2519954748046652No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGAGTACCCCTACCAAGTGGT32530.24916148314272826No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT32050.24548495341913434No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGTACCGCTAC28250.21637909310734932No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGTAACACCGTACCCCTACCA26250.20106021925904138No Hit
ACGATCCATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC25820.19776666138165516No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCGCTAC24860.19041360193446738No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACTAAACACCGTACCCCTACC23130.17716277605568104No Hit
GATCGATAACGCATTTTTTGTGGAAAGGACGAAACACCGTACCCCTACCA22550.17272030263967175No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCGCTAC21460.16437151639234393No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT19870.15219301168293914No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCCCTAC19790.15158025672900682No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACAGGGTACCCCTACCAAGTGGT19600.15012496371341758No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC19060.14598886777437442No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAAGTGGT18790.14392081980485288No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTGGAAACACCGTACCCCTAC18220.1395549407580851No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACCAAACACCGTACCCCTACCA17880.13695073220387274No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAAGTGGT16570.12691686983323108No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGTCGAAACACCGTACCGCTAC16160.12377650069432795No Hit
CGATCGATACAGGTATACTTGTGGAAAGGAGGAAACACCGTACCCCTACC15820.1211722921401156No Hit
TAACAAAGGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAAGTGGT14570.11159799598492316No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGGAAACACCGTACCGCTAC14180.10861081558450313No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT13780.10554704081484154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)