FastQCFastQC Report
Thu 24 Oct 2024
H5GM2BGYW_n01_SRS_S2_WT_clone2_Rep2_ox_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GM2BGYW_n01_SRS_S2_WT_clone2_Rep2_ox_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11514727
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC186440.16191439015445178RNA PCR Primer, Index 5 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATGTCGTATGC115350.10017606149064584RNA PCR Primer, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)