FastQCFastQC Report
Thu 24 Oct 2024
H5GM2BGYW_n01_SRS_S2_WT_clone1_Rep2_ox_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GM2BGYW_n01_SRS_S2_WT_clone1_Rep2_ox_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11997780
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1503011.2527400902500296RNA PCR Primer, Index 4 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC352630.2939127071841624RNA PCR Primer, Index 4 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC269450.22458321456136052RNA PCR Primer, Index 4 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC234810.19571120657321606RNA PCR Primer, Index 4 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC224490.18710961527882658RNA PCR Primer, Index 4 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC217330.18114184457457963RNA PCR Primer, Index 4 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC150360.125323184789186RNA PCR Primer, Index 4 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC131590.10967862387875091RNA PCR Primer, Index 4 (100% over 44bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC123150.10264398913799053RNA PCR Primer, Index 4 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)