FastQCFastQC Report
Thu 24 Oct 2024
H5GM2BGYW_n01_SRS_S2_Hen1_clone3_Rep2_ox_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GM2BGYW_n01_SRS_S2_Hen1_clone3_Rep2_ox_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11456818
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1493941.3039746289065604RNA PCR Primer, Index 3 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC331260.2891378740589228RNA PCR Primer, Index 3 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC272490.2378409083569277RNA PCR Primer, Index 3 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC237440.2072477715889351RNA PCR Primer, Index 3 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC222610.1943035142916646RNA PCR Primer, Index 3 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC217810.19011386931345162RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAGCTCGTATGC184950.16143225806676864RNA PCR Primer, Index 3 (98% over 50bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC137940.12039992256139531RNA PCR Primer, Index 3 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC135770.11850585389416154RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCGCGTATGC132670.11580004151239898RNA PCR Primer, Index 3 (98% over 50bp)
TAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC131280.11458679015412482RNA PCR Primer, Index 3 (100% over 44bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC130400.11381868857478576RNA PCR Primer, Index 3 (100% over 44bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG123430.10773497492933902RNA PCR Primer, Index 3 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)