H5GM2BGYW_n01_SRS_S2_Hen1_clone2_Rep2_ox_2.fastq.gz FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content

Basic Statistics

Measure	Value
Filename	H5GM2BGYW_n01_SRS_S2_Hen1_clone2_Rep2_ox_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10232298
Sequences flagged as poor quality	0
Sequence length	76
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	160185	1.5654841170575757	RNA PCR Primer, Index 2 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	26814	0.26205257118195735	RNA PCR Primer, Index 2 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCGCGTATGC	26535	0.25932591095372715	RNA PCR Primer, Index 2 (98% over 50bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	19587	0.19142327559263814	RNA PCR Primer, Index 2 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	18642	0.1821878135292776	RNA PCR Primer, Index 2 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	17785	0.17381237333001834	RNA PCR Primer, Index 2 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	16985	0.16599399274727927	RNA PCR Primer, Index 2 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	16137	0.1577065093295758	RNA PCR Primer, Index 2 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	15841	0.15481370851396237	RNA PCR Primer, Index 2 (100% over 44bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	14652	0.1431936403728664	RNA PCR Primer, Index 2 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	14227	0.13904012568828625	RNA PCR Primer, Index 2 (100% over 44bp)
TTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	14212	0.13889353105235988	RNA PCR Primer, Index 2 (100% over 44bp)
CTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	14059	0.13739826576591105	RNA PCR Primer, Index 2 (100% over 44bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	13569	0.13260950765898336	RNA PCR Primer, Index 2 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAGCTCGTATGC	12669	0.12381382950340186	RNA PCR Primer, Index 2 (98% over 50bp)
TGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	12009	0.11736366552264213	RNA PCR Primer, Index 2 (100% over 44bp)
ATGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	10421	0.10184418006590505	RNA PCR Primer, Index 2 (100% over 44bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	10395	0.10159008269696602	RNA PCR Primer, Index 2 (100% over 50bp)

Adapter Content

Adapter graph