FastQCFastQC Report
Thu 24 Oct 2024
H5GM2BGYW_n01_SRS_S2_Hen1_clone1_Rep2_ox_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GM2BGYW_n01_SRS_S2_Hen1_clone1_Rep2_ox_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12063724
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1528101.2666901198999578RNA PCR Primer, Index 1 (100% over 50bp)
ACAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC308380.2556258747298927RNA PCR Primer, Index 1 (100% over 44bp)
AAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC243210.20160441336356835RNA PCR Primer, Index 1 (100% over 44bp)
ATTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC212040.17576662065544604RNA PCR Primer, Index 1 (100% over 44bp)
TCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC203800.16893622566298763RNA PCR Primer, Index 1 (100% over 44bp)
TGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC199340.16523919148017643RNA PCR Primer, Index 1 (100% over 44bp)
AATATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC199150.16508169450826296RNA PCR Primer, Index 1 (100% over 44bp)
ATAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC195230.16183228329825847RNA PCR Primer, Index 1 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAGCTCGTATGC173090.1434797414131822RNA PCR Primer, Index 1 (98% over 50bp)
TTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC157600.1306395935450778RNA PCR Primer, Index 1 (100% over 44bp)
ACTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC153280.1270586097626239RNA PCR Primer, Index 1 (100% over 44bp)
TCAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC143160.11866982367965315RNA PCR Primer, Index 1 (100% over 44bp)
TTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC136260.11295019680490039RNA PCR Primer, Index 1 (100% over 44bp)
TGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC134940.11185600731581724RNA PCR Primer, Index 1 (100% over 44bp)
CTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC126490.10485153672282291RNA PCR Primer, Index 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)