FastQCFastQC Report
Thu 14 Apr 2022
H5GLJDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GLJDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045987616
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGG737602023.605114782864844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGGAC179947810.8795156363253374Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACCCCAGGTCAGTCGGGA155362890.7593540096969972Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGGA127076640.6211017066097433Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCGGCGACGCATCATTCA99120820.4844644181854129Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGGACTAC93292730.4559789574014704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTCGTGCCTCCGGC88103280.4306149231354878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGCTCTGGATACATTAGCA87220620.42630082077681547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGGACT82300010.40225077295873524Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCTCCTTTTGGCTCCAA68371840.3341752387224616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCCCTCGACCTATTCTCAA56396580.27564477692322453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGGACTA50750820.2480504750034616Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGTGCCGGCGACGCATCAT45122530.22054155971978276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGCGACGCATCATTCAA44895110.21943001829000316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCCTTCCCCGGGCGTC41759400.2041038746932474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCCGGCGACGCATCATTC40012520.19556579759864978Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGCCGGGTCGTGCCTCCGG37893740.18521001644225005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCCGGGCCGATCCGGGCGG37489390.18323370926991964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGACCCCAGGTCAGTCGGGACT37457270.18307671907237977Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGAGAGCGGGCCGCCG36585380.17881525632851142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30228390.14774473590948656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGCCGGCGACGCATCATT29981030.14653573543428525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGTCGGGACTACCC29951410.14639096427453646No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGCGACCCCAGGTCAGTCGGGAC26720260.13059834669106815Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATGCATTGGATGGATGCC23761960.11613931489211907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGGCGCCGTCAACGAGTC21841880.10675470285935494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTCGTGCCTCCGGCGC21521950.10519100815515395Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGGCCGGGTCGTGCCTCCG20948790.10238962267501818Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGTGCCGGCGACGCATCATTCAAA20786800.10159787790230693Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)