FastQCFastQC Report
Thu 14 Apr 2022
H5GLJDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GLJDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045987616
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGTCA1284947236.280327505169024No Hit
GACTACGT1139539645.569631170240671No Hit
CTAGCGAG1121329425.480626623695067No Hit
TCTATATC1094287795.34845754413403No Hit
AGAGTCGC1091743625.33602262038325No Hit
TTAGCTCG1075181655.255074085453311No Hit
ACGGACAT1035039825.058876270343955No Hit
TCGCTTCG1020165544.9861765145698715No Hit
CGGAGCAC999180344.883608933828463No Hit
CGATCACC952224044.654104612136616No Hit
GACCTATT939425424.591549883555111No Hit
AACAAGAT932715014.5587519822016365No Hit
GTATGCTC931724114.553908844382761No Hit
ACTTAGGA903427374.415605270212936No Hit
CGTGCAGA843497784.1226925002071955No Hit
TATCGATA840208774.106617085213091No Hit
GATCTTGA838801084.099736838289837No Hit
TTCAGGTG777388313.799574855295703No Hit
CCCAATAG759721433.713225945547463No Hit
GTGTCGCT690201303.373438307262951No Hit
GGGGGGGG443435212.16734063555544No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)