FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181624
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT4659865.075202382497912No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG2547222.774258671450715No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA1782691.9415846259877338No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG1662631.8108234447413658No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG1611821.755484650645681No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1514311.6492833947458534No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG1413921.5399454388461127No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1184041.2895757874641784No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC1107471.2061809544803839No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA1086161.1829715527449174No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG1072881.168507880523097No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT932721.015855147194004No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG930631.0135788614301782No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG709970.7732510065757431No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC603040.6567901277595336No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT572210.6232121899132441No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC571180.6220903840104975No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG527100.57408144790072No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC518910.5651614572759678No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG510400.5558929444290029No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG484450.5276299704714547No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC478290.5209209176938633No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG475860.5182743270689368No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG461920.503091827763803No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT458960.499867997208337No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT456470.49715605866674567No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT418380.45567102290400907No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA400460.43615377846010683No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA384320.41857518887726175No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG379450.4132711163079647No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT379260.41306418123852595No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG375570.40904528436363763No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA365140.3976856381833976No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT354170.38573786075317396No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG330560.3600234555455549No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT319960.3484786569347645No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG313090.34099632047663897No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC303740.3308129367963663No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT301610.32849308575476405No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA293710.319888943393892No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG288200.31388782638016977No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG286770.3123303676996575No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG279000.3038678124915592No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT278660.3034975076304584No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC277390.3021143100610524No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT269800.2938477986029487No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT262490.2858862440892809No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA260400.28360995832545527No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT255130.27787023297839253No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG254080.2767266444367576No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG247890.26998491770083377No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA240650.262099602423275No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG232210.25290732881241923No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT220850.24053478992387403No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT219880.23947833193779228No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT219650.239227831590577No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG204220.22242252568826604No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT203750.22191063367439137No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC198390.2160728864523313No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC184280.20070523471664709No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC182240.1984834055500421No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC176600.19234070138354611No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC174700.19027135068915912No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT171690.1869930635364724No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT166820.1816889909671753No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT163340.17789881180061393No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG162300.17676611457842317No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG160760.1750888513840253No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTG160200.17447893749515336No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT159900.1741521979118291No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG157400.17142936805079362No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT147200.16032022221776887No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG146840.15992813471777978No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG144990.15791324062061352No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG144640.15753204444006855No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT138110.15042001284304388No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG131000.142676284718259No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC129160.14067228194053688No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC127650.13902769270447146No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT124550.13565138367678745No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC120260.13097900763525058No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC119000.1296067013852887No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC118760.12934530971862931No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT118250.12878985242697805No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT116720.12712348055202433No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG114090.12425906353821503No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG113740.12387786735767005No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT112980.12305012707991528No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTTCCAA112660.12270160485770275No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT108010.11763714131617674No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC106220.11568759513567535No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG104800.11414102777460719No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT104200.11348754860795868No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG103500.11272515624686874No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT99640.10852110694142995No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCATCTGG94780.103227925691577No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT93160.10146353194162602No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG92120.10033083471943526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)