FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_eRNA_lib02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_eRNA_lib02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13354283
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT135061010.113684126658091No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG7477265.59914747950152No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG4592413.4389042077362No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG4323613.237620469777374No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG3934972.946597731978572No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG3459482.590539679292404No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT3217882.4096239386270306No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG2881262.1575549956519566No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT2643031.979162789945368No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG2605891.9513514877586464No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT2506911.8772329446665164No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT2364621.7706828588251424No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT2364051.7702560294700957No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT2076011.5545649287198722No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1932721.4472660194485918No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1865041.3965856497125304No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1726451.2928062105618099No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG1588991.1898729418868836No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT1544991.1569247109710046No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG1445751.0826114737870989No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1438711.0773397568405583No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT1375941.0303361101453368No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG1323480.9910528330124501No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG1275810.9553564201088145No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG1161380.8696685550246315No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGACTACGACCTGAATGC1154830.8647637615587449No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACTACGACCTGAATGCT1104980.8274349135779134No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT1104900.827375007703521No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG1057920.792195282966521No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG936880.7015576950106569No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG901210.6748471632658976No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT899940.6738961575099165No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG819680.6137955890256331No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG802010.6005638790191881No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT788620.5905371332927422No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGACTACGACCTGAATGC786510.5889571158556397No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT761240.5700343477819064No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT751710.5628980604948989No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT748510.5605018255191986No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGACTACGACCTGAATGC685840.5135730611669679No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT665250.4981547867451963No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT617030.4620465209551123No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG605210.45319542801361934No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT551010.4126091981126954No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT550720.4123920393180225No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG549920.4117929805740975No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG549620.41156833354512556No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT522780.39146991268643927No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG490780.3675075629294362No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG477970.3579151347923359No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG443570.33215560880355766No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG434020.325004345047952No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGACTACGACCTGAATG433370.3245176098185129No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG385680.2888062204462793No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACGAACT381620.2857659973208595No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT373520.2797005275386181No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG361160.2704450699449757No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG355210.265989570537033No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG351360.2631066003318935No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGACTACGACCTGAATGC348750.26115217117983797No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT348470.26094250061946417No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT331090.24792794940769192No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGACTACGACCTGAATGC319260.23906936823189984No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACGCCTACCTACTG299420.22421271138255797No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT295390.22119495296003536No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCTACCTACTG290720.2176979475423727No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT284790.2132574246030281No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCTACCTACTG280420.20998506621433738No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG277430.2077460841589174No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG275930.2066228490140579No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACGCCTACCTACTG269620.20189777317134883No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG264350.19795147369574242No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG261510.19582481515480837No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT251260.18814937499826836No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT248010.18571569885107272No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACGAACT245180.18359652854443778No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGACTACGACCTGAATGC241990.18120778180303654No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT237540.1778755175399533No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGACTACGACCTGTATGC235910.17665493534920595No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCTACCTACTG233160.1745956709169635No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG226140.16933893043902096No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT224540.16814081295117078No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT222720.16677795430874123No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGACTACGACCTGAATGCT222620.16670307196575063No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG221820.16610401322182552No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGACTACGACCTGAATGC220720.16528030744892855No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT215910.16167846675107903No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGACTACGACCTGAATGCT206810.15486417353893128No Hit
GATCGCTCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG198990.14900837431706368No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCTACCAACTG187820.14064401660500978No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG176710.1323245882987503No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG174840.13092428848482543No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCTACCTACTG173770.13012304741482564No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG169950.1272625419125834No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCGCTACCATCTGG167610.12551029508660255No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT166690.12482137753108871No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACTACGACCTGATTGCT155060.11611256104127791No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG154180.11545359642296034No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACGCCTACCTACTG154000.1153188082055772No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACGAACT153750.1151316023481006No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGACTTCGACCTGAATG153150.1146823082901568No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGACTACGACCTGAATGC147370.11035410886529812No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGACTACGACCTGAATG145930.10927580312623299No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACAGCGTACCCCTACCAACTGGT142770.10690952108772893No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT141790.10617567412642072No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCATCTGG138380.10362218623044007No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGACTACGACCTGATTGCT134060.10038726901324467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)