FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_eRNA_lib01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_eRNA_lib01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15161671
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT11927877.867120978947504No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT11189587.380175971368855No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT6103324.02549296842017No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT5448313.593475943383813No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT5181363.417406959958437No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG5053733.3332275842154866No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG4635073.0570970706329135No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT4413802.911156692425261No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT4173542.752691309552885No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG4027082.6560924584104217No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT3684842.430365360124224No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT3355962.213449955483139No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT3130142.06450858879605No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG3038792.00425797393968No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG2970441.959177190957382No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT2908561.9183637476370512No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG2405381.5864873997068003No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT2297881.5155849246432005No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT2279351.5033633166159588No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT2226221.4683210049868514No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT2002951.321061510964062No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT1861251.2276021554616243No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG1780201.174144987053208No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG1740201.1477626707504733No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1482770.9779726786051485No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT1446700.9541824248791575No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT1364050.8996699638186318No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACGAACT1296330.855004702318102No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG1289060.85020971633008No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT1226410.8088884134209218No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG1217800.8032096198367582No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG1182530.7799470124368217No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG1096710.7233437528093045No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG1007700.664636503456644No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG980350.6465975946846493No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG911920.6014640470697459No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT871950.5751015175042382No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT867620.5722456317644671No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT764980.5045486081316499No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT757020.49929852718740564No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT736360.48567206081704317No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACGAACT694730.45821466512497205No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG648380.4276441561091782No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG581900.3837967464140331No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT568750.3751235599295091No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG544980.359445868466609No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT527070.34763318634205953No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG525160.34637343073860394No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG512900.33828725079181576No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG478790.3157897305646587No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACCAACT440420.2904824936512605No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG402460.2654456754799652No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG393960.25983943326563413No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG375270.24751229597318133No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGTACCCCTACGAACT373220.24616020226266616No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG359410.237051707559147No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACGAACT358160.23622726017468654No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG347770.2293744535150512No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT281250.1855006615036034No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG280300.18487408149141343No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACCAACT279360.18425409705829918No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT252020.16622178386538003No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG246860.16281846506232722No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGTACCCCTACCAACT242750.16010768206222126No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCAACTGG231150.15245681033442818No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAAGACCGTACCCCTACGAACT218630.14419914533167222No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACTAACT205550.13557212790067796No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACGCCTACCTACTG201140.13266347752830146No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGTACCCCTACCAACTGGT197530.13028247348197966No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCAACTGG187830.1238847617785665No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCGCTACCATCTGG186930.12329115966175495No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAAGTGGT184790.12187970573955866No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG183990.12135205941350397No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACGCCTACCTACTG175270.1156007144595078No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTAGCCCTACGAACT174720.11523795761034519No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT164940.10878748127432657No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)