FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_THP1_T0_R2__CD235_High_R1__CD235_Low_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_THP1_T0_R2__CD235_High_R1__CD235_Low_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23709035
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA246579910.400250368688562No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA15353266.475700086486016No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC11943825.037666020569795No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT10881784.589718645233769No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC5742582.422106171761103No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT5599072.361576504484472No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC5180892.185196487330674No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA4956332.090481540054245No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC4914672.0729101796003087No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA4839802.041331500839237No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC4742842.0004356988801946No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT4452401.8779338762627833No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT4023471.6970197226500363No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA3666911.54662979745907No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT3649291.5391980314677505No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA3573751.5073367600157492No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC3493431.4734593795150246No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT3340421.4089228009490897No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA3285571.3857881605050564No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT3268431.378558848978881No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT3065871.2931230646882084No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT3034591.279929782043006No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA2992401.2621348781171398No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT2960191.248549339945721No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC2649521.117514905182771No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC2429071.0245334742641359No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT2390841.0084088196757057No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT2094740.8835197214901409No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC1940890.8186288476102043No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC1908950.8051571900754291No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT1876780.7915885231094391No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC1756510.7408610261868523No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC1481450.624846182056756No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC1470140.6200758487218059No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1409850.5946467243394765No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT1330690.5612586087961825No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTTCCAA1301320.548870926210198No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC1276050.5382125421806496No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC1269150.5353022592442079No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT1263180.5327842318339823No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1223980.5162504505139075No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1167250.4923228634147277No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1163140.4905893470569342No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC1127190.4754263511779371No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT1102290.46492402579860376No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC1066520.4498369503440355No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT1065650.44947000162596246No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT1008140.42521342602092405No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA979760.4132433057692985No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC968290.40840548761263373No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA947490.39963246078973697No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC921850.3888180181099737No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC900130.37965695356221796No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT884140.372912689192116No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTACCAA864820.36476389697007916No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT863810.3643378990330058No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT856140.36110284539206255No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC825620.34823011565000433No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT790770.3335310779202949No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT778970.3285540723188438No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT774250.3265632700782634No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA749320.3160482912948587No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT728060.30708124560953237No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC727090.3066721188778877No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT688440.29037031663245677No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTAGCAAC670030.28260534433392165No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA668130.2818039620760609No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT662610.2794757357269075No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT659910.2783369293604738No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA649360.27388714892866795No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT574840.24245609321509712No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC529900.22350129391601137No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC502350.2118812511770302No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT489780.2065794748710776No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTACCAAC487920.20579496381864548No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC477540.20141688600991142No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT461620.1947021462493096No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT433330.18276998620989846No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC433110.1826771945800409No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT395650.16687731069611225No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC394350.16632899651968122No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC385900.16276495437287936No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC384960.16236848104530616No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACGAACT382310.16125076368565822No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC376740.15890144832971903No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT376070.1586188556387892No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGTACCCCTTCCAA369770.15596164078377717No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA357030.15058816185475285No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT352060.14849191458024336No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT348000.14677948722923562No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC330560.13942364166234517No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC306940.1294611948567287No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTAGCAAC280530.11832198147246399No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT280010.11810265580189155No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT274800.11590518129481017No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACGAACT271950.11470310790801903No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC263970.1113373024250038No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC254950.10753284560084374No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC252760.10660914710362526No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT251870.10623376278283785No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC249200.10510760982047561No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT237970.10037101889638275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)