FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_THP1_PMA_R2__CD41_High_R1__CD41_Low_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_THP1_PMA_R2__CD41_High_R1__CD41_Low_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20895358
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT374408117.918242893948026No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG15978897.647100375116808No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG10806555.1717467582991405No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG9597714.593225921278783No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT7857193.760256225330047No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT7633303.653108025236993No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG7474463.5770911414870232No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG6369713.048385196367538No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT5369512.569714287737975No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG5313052.542693932307836No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG4751102.273758602269461No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG3179971.5218547583630777No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG3144331.5047983384634998No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG2878931.3777844820844898No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG2718431.3009731635131594No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG2716631.3001117281646957No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG2482411.188019846321848No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG2211121.0581871820525879No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG2096151.0031653920454484No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG1991360.9530154975090639No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1961500.9387252422284412No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT1959610.9378207351125547No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG1918900.9183379389814714No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG1895460.9071201364437019No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT1590450.7611499166465585No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG1575210.753856430696234No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG1550800.7421744102206815No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1365400.653446569328939No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1210300.579219556802999No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT1087590.5204935947974665No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1069030.5116112392044204No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG1018990.48766333651713456No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG996900.4770916104907128No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG983050.47046334405947965No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG962070.46042283649794374No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG944680.45210041388139893No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG884700.4233954737698201No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTG771390.36916811858404147No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG735300.3518963398473479No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG724340.34665115572559224No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT665230.31836257603243745No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT658680.31522790851441745No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG656490.3141798288404535No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG631440.3021915202410028No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG615000.2943237440583693No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG588830.28179943124209694No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCATCTGG584390.2796745573825536No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG549640.2630440694052717No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG495750.23725365222266112No Hit
GATCGCTCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG493530.23619121529288947No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG488550.23380791082880706No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG483700.23148682113989147No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG468980.2244421942902342No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT467100.22354247292628343No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG464780.2224321784771527No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG460280.2202785901059939No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT436820.20905121606435267No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCATCTGG431350.20643340975541072No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG429190.2053996873372545No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG404620.19364109483072747No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT388240.18580203315970945No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT380260.1819830031148545No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT359070.1718419947626645No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG358420.17153092088683045No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG354330.1695735483450439No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT333940.15981540014772658No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG285840.13679593333600698No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG278410.133240119647627No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCAACTGG268550.12852136823882127No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG258480.12370211603936146No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCGCTACCATCTGG246060.1177582121349632No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCGCTACCATCTGG232080.11106773092856319No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG231070.11058436998303642No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT223580.106999841783041No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG218790.10470746660574085No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG218250.1044490360012018No Hit
GATCGCACGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG215670.10321431200173742No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG209900.10045293313471824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)