FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_K562_T0_R1__K562_T0_R2__THP1_T0_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_K562_T0_R1__K562_T0_R2__THP1_T0_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935692
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA8177448.230367849566996No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA7713277.763193545049504No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC5433595.468758492111068No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT3941723.967232478623532No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA2601532.618368202234932No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC2484662.5007417701756456No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC2424212.439900512213945No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC2263312.277959099376269No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA2115082.1287696921361894No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT2086192.099692703839853No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC2014792.0278305728478703No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA1893611.90586624464607No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA1751681.7630176136699889No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA1707991.7190448335153707No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT1678421.6892834439714919No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC1530191.5400940367314124No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1478291.487858117985139No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT1301491.3099137936240375No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1296381.3047707195432385No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1291521.299879263568154No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT1248301.2563795254522785No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT1162391.1699134796046415No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT1144551.1519580115808743No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT1120201.1274504080843084No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC1077381.0843532589375757No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTTCCAA990700.9971122293243391No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT961620.9678440112676601No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC913920.9198352766973856No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC901930.9077676723473312No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA800230.8054094269427837No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC779580.7846257714107885No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT763780.7687235071296493No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC731120.7358521177991427No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT695170.6996694341974369No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA673650.6780101476575562No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC644980.6491545832942486No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC601640.6055340684876303No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTACCAA564870.5685260774991817No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC556570.5601723563894694No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC541870.5453772117734729No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC539840.5433340727550733No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT503720.5069802888414818No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT499190.50242096876594No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC483700.48683071093588653No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT455090.45803553491795035No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC453030.4559622017268651No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA449170.4520772181746374No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT447630.4505272506434378No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA445700.44858475886732396No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT438130.4409657626262972No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT435530.4383489343268692No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC430790.43357825504252745No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT413280.41595492291830305No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC394830.3973855067165931No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC376860.37929919727785444No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC374060.3764810744938551No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT368340.37072405223511357No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT350050.3523156716210607No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGTACCCCTTCCAA332810.3349640870510076No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTAGCAAC310390.3123989753305557No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT310280.3122882633640415No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC307110.30909774578358507No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT304930.306903635901757No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT298230.3001602706686157No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT294610.29651684049787375No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT281440.28326159868884826No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT276440.27822923657456367No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT268500.27023784553707986No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT257000.25866341267422543No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC249940.2515577173688556No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT247740.24934347803857046No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC233600.23511195797937373No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC225430.2268890782846328No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTACCAAC220090.22151451554657692No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC195760.19702704149846834No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT184980.18617726878007088No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT181110.18228222050361464No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC179550.18071212352395788No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT178560.17971571582532953No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC173240.17436128253573077No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTACCAA171310.17241879075961694No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC171260.17236846713847412No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA170500.17160354809710285No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT167140.16822180075630364No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT155180.15618439057893502No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC152930.15391982762750697No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTACCAA145130.1460693427292231No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC144390.145324553136309No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACGAACT143970.14490183471870907No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA142510.143432384981338No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC133330.13419296813951156No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT130250.13109303307711231No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT130100.13094206221368376No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC126850.12767102683939882No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTAGCAAC126210.1270268844887704No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT118550.11931730572968646No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC116660.11741507285048691No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC116470.1172238430901441No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT115560.1163079531853443No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACGAACT109120.10982627078214581No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC104670.10534746850043258No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT102190.10285141689174745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)